論文 - 詳細
| RRC ID | 36174 |
|---|---|
| 著者 | Zaessinger S, Zhou Y, Bray SJ, Tapon N, Djiane A. |
| タイトル | Drosophila MAGI interacts with RASSF8 to regulate E-Cadherin-based adherens junctions in the developing eye. |
| ジャーナル | Development |
| Abstract |
Morphogenesis is crucial during development to generate organs and tissues of the correct size and shape. During Drosophila late eye development, interommatidial cells (IOCs) rearrange to generate the highly organized pupal lattice, in which hexagonal ommatidial units pack tightly. This process involves the fine regulation of adherens junctions (AJs) and of adhesive E-Cadherin (E-Cad) complexes. Localized accumulation of Bazooka (Baz), the Drosophila PAR3 homolog, has emerged as a critical step to specify where new E-Cad complexes should be deposited during junction remodeling. However, the mechanisms controlling the correct localization of Baz are still only partly understood. We show here that Drosophila Magi, the sole fly homolog of the mammalian MAGI scaffolds, is an upstream regulator of E-Cad-based AJs during cell rearrangements, and that Magi mutant IOCs fail to reach their correct position. We uncover a direct physical interaction between Magi and the Ras association domain protein RASSF8 through a WW domain-PPxY motif binding, and show that apical Magi recruits the RASSF8-ASPP complex during AJ remodeling in IOCs. We further show that this Magi complex is required for the cortical recruitment of Baz and of the E-Cad-associated proteins α- and β-catenin. We propose that, by controlling the proper localization of Baz to remodeling junctions, Magi and the RASSF8-ASPP complex promote the recruitment or stabilization of E-Cad complexes at junction sites. |
| 巻・号 | 142(6) |
| ページ | 1102-12 |
| 公開日 | 2015-3-15 |
| DOI | 10.1242/dev.116277 |
| PII | dev.116277 |
| PMID | 25725070 |
| PMC | PMC4360174 |
| MeSH | Adherens Junctions / metabolism Adherens Junctions / physiology* Animals Blotting, Western Cadherins / metabolism* Carrier Proteins / metabolism* Drosophila Drosophila Proteins / metabolism* Eye / embryology* Immunohistochemistry Immunoprecipitation Intracellular Signaling Peptides and Proteins / metabolism Morphogenesis / physiology* Multiprotein Complexes / metabolism Nucleoside-Phosphate Kinase / metabolism* Plasmids / genetics Two-Hybrid System Techniques |
| IF | 5.611 |
| 引用数 | 12 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |