RRC ID 36199
Author Oda Y, Kimura M, Kose S, Fasken MB, Corbett AH, Imamoto N.
Title The Schizosaccharomyces pombe Hikeshi/Opi10 protein has similar biochemical functions to its human homolog but acts in different physiological contexts.
Journal FEBS Lett.
Abstract Human Hikeshi (HsHikeshi) is a nuclear import carrier for Hsp70s and is required for cell survival after heat shock. The Hikeshi homolog in Schizosaccharomyces pombe (SpHikeshi/Opi10) localizes to the nuclear rim, interacts with the Hsp70 homolog Ssa2, and mediates its nuclear import in a reconstituted mammalian nuclear transport system. However, SpHikeshi/Opi10 is not required for heat stress response and survival after heat stress. Instead, SpHikeshi/Opi10 is required for the normal expression of stress response genes under optimal conditions and for cell growth during glucose deprivation. Here, the functions of SpHikeshi/Opi10 are discussed and compared to the functions of HsHikeshi.
Volume 588(10)
Pages 1899-905
Published 2014-5-21
DOI 10.1016/j.febslet.2014.04.018
PII S0014-5793(14)00304-4
PMID 24768994
MeSH Active Transport, Cell Nucleus Amino Acid Sequence Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Nucleus / metabolism Gene Expression Regulation, Fungal HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism HeLa Cells Heat-Shock Response* Humans Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Fluorescence Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Protein Binding Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Homology, Amino Acid Temperature Transcriptome
IF 2.675
Times Cited 2
Yeast spa73g12 and spa101a06