| RRC ID |
36249
|
| 著者 |
Shimaji K, Konishi T, Tanaka S, Yoshida H, Kato Y, Ohkawa Y, Sato T, Suyama M, Kimura H, Yamaguchi M.
|
| タイトル |
Genomewide identification of target genes of histone methyltransferase dG9a during Drosophila embryogenesis.
|
| ジャーナル |
Genes Cells
|
| Abstract |
Post-translational modification of the histone plays important roles in epigenetic regulation of various biological processes. Among the identified histone methyltransferases (HMTases), G9a is a histone H3 Lys 9 (H3K9)-specific example active in euchromatic regions. Drosophila G9a (dG9a) has been reported to feature H3K9 dimethylation activity in vivo. Here, we show that the time required for hatching of a homozygous dG9a null mutant and heteroallelic combination of dG9a null mutants is delayed, suggesting that dG9a is at least partially responsible for progression of embryogenesis. Immunocytochemical analyses of the wild-type and the dG9a null mutant flies indicated that dG9a localizes in cytoplasm up to nuclear division cycle 7 where it is likely responsible for di-methylation of nucleosome-free H3K9. From cycles 8-11, dG9a moves into the nucleus and is responsible for di-methylating H3K9 in nucleosomes. RNA-sequence analysis utilizing early wild-type and dG9a mutant embryos showed that dG9a down-regulates expression of genes responsible for embryogenesis. RNA fluorescent in situ hybridization analysis further showed temporal and spatial expression patterns of these mRNAs did not significantly change in the dG9a mutant. These results indicate that dG9a controls transcription levels of some zygotic genes without changing temporal and spatial expression patterns of the transcripts of these genes.
|
| 巻・号 |
20(11)
|
| ページ |
902-14
|
| 公開日 |
2015-11-1
|
| DOI |
10.1111/gtc.12281
|
| PMID |
26334932
|
| MeSH |
Animals
Cell Nucleus / metabolism
Cytoplasm / metabolism
Drosophila Proteins / genetics
Drosophila Proteins / metabolism
Drosophila melanogaster
Embryonic Development / genetics
Epigenesis, Genetic
Epigenomics
Histone Methyltransferases
Histone-Lysine N-Methyltransferase / genetics*
Histone-Lysine N-Methyltransferase / metabolism*
Histones / metabolism
Methylation
Molecular Sequence Data
Protein Processing, Post-Translational
Transcriptome
|
| IF |
1.655
|
| 引用数 |
8
|
|
WOS 分野
|
GENETICS & HEREDITY
CELL BIOLOGY
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| リソース情報 |
| ショウジョウバエ |
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