| RRC ID |
3624
|
| Author |
Penkner A, Tang L, Novatchkova M, Ladurner M, Fridkin A, Gruenbaum Y, Schweizer D, Loidl J, Jantsch V.
|
| Title |
The nuclear envelope protein Matefin/SUN-1 is required for homologous pairing in C. elegans meiosis.
|
| Journal |
Dev Cell
|
| Abstract |
We identify a highly specific mutation (jf18) in the Caenorhabditis elegans nuclear envelope protein matefin MTF-1/SUN-1 that provides direct evidence for active involvement of the nuclear envelope in homologous chromosome pairing in C. elegans meiosis. The reorganization of chromatin in early meiosis is disrupted in mtf-1/sun-1(jf18) gonads, concomitant with the absence of presynaptic homolog alignment. Synapsis is established precociously and nonhomologously. Wild-type leptotene/zygotene nuclei show patch-like aggregations of the ZYG-12 protein, which fail to develop in mtf-1/sun-1(jf18) mutants. These patches remarkably colocalize with a component of the cis-acting chromosomal pairing center (HIM-8) rather than the centrosome. Our data on this mtf-1/sun-1 allele challenge the previously postulated role of the centrosome/spindle organizing center in chromosome pairing, and clearly support a role for MTF-1/SUN-1 in meiotic chromosome reorganization and in homolog recognition, possibly by mediating local aggregation of the ZYG-12 protein in meiotic nuclei.
|
| Volume |
12(6)
|
| Pages |
873-85
|
| Published |
2007-6-1
|
| DOI |
10.1016/j.devcel.2007.05.004
|
| PII |
S1534-5807(07)00199-2
|
| PMID |
17543861
|
| MeSH |
Animals
Animals, Genetically Modified
Apoptosis
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / physiology*
Cell Nucleus / metabolism
Chromosome Pairing*
DNA Replication
Gonads / metabolism
In Situ Hybridization, Fluorescence
Meiosis*
Nuclear Envelope / metabolism*
Protein Transport
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / physiology*
Recombination, Genetic*
|
| IF |
10.092
|
| Times Cited |
130
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm1458 |
