RRC ID 3633
著者 Stitzel ML, Cheng KC, Seydoux G.
タイトル Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition.
ジャーナル Curr Biol
Abstract BACKGROUND:Successful transition from oocyte to zygote depends on the timely degradation of oocyte proteins to prepare for embryonic development. In C. elegans, degradation of the oocyte protein MEI-1 depends on MBK-2, a kinase that phosphorylates MEI-1 shortly after fertilization during the second meiotic division.
RESULTS:Here we report that precise timing of MEI-1 phosphorylation depends on the cell cycle-regulated release of MBK-2 from the cortex. Prior to the meiotic divisions, MBK-2 is tethered at the cortex by EGG-3, an oocyte protein required for egg activation (see [1], accompanying paper in this issue). During the meiotic divisions, EGG-3 is internalized and degraded in an APC/C (anaphase-promoting complex/cyclosome)-dependent manner. EGG-3 internalization and degradation correlate with MBK-2 release from the cortex and MEI-1 phosphorylation in the cytoplasm. In an egg-3 mutant, MEI-1 is phosphorylated and degraded prematurely.
CONCLUSION:We suggest that successful transition from an oocyte to a zygote depends on the cell cycle-regulated relocalization of key regulators from the cortex to the cytoplasm of the egg.
巻・号 17(18)
ページ 1545-54
公開日 2007-9-18
DOI 10.1016/j.cub.2007.08.049
PII S0960-9822(07)01905-7
PMID 17869113
MeSH Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Amino Acid Sequence Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology* Gene Expression Regulation, Developmental Green Fluorescent Proteins / analysis Meiosis Molecular Sequence Data Oocytes / enzymology* Oocytes / growth & development Phosphorylation Protein Tyrosine Phosphatases / chemistry Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / physiology* Protein-Tyrosine Kinases / analysis Protein-Tyrosine Kinases / metabolism* Recombinant Fusion Proteins / analysis Ubiquitin-Protein Ligases / metabolism Zygote / enzymology Zygote / growth & development Zygote / metabolism
IF 9.601
引用数 44
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1191