論文 - 詳細
RRC ID | 3633 |
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著者 | Stitzel ML, Cheng KC, Seydoux G. |
タイトル | Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition. |
ジャーナル | Curr Biol |
Abstract |
BACKGROUND:Successful transition from oocyte to zygote depends on the timely degradation of oocyte proteins to prepare for embryonic development. In C. elegans, degradation of the oocyte protein MEI-1 depends on MBK-2, a kinase that phosphorylates MEI-1 shortly after fertilization during the second meiotic division. RESULTS:Here we report that precise timing of MEI-1 phosphorylation depends on the cell cycle-regulated release of MBK-2 from the cortex. Prior to the meiotic divisions, MBK-2 is tethered at the cortex by EGG-3, an oocyte protein required for egg activation (see [1], accompanying paper in this issue). During the meiotic divisions, EGG-3 is internalized and degraded in an APC/C (anaphase-promoting complex/cyclosome)-dependent manner. EGG-3 internalization and degradation correlate with MBK-2 release from the cortex and MEI-1 phosphorylation in the cytoplasm. In an egg-3 mutant, MEI-1 is phosphorylated and degraded prematurely. CONCLUSION:We suggest that successful transition from an oocyte to a zygote depends on the cell cycle-regulated relocalization of key regulators from the cortex to the cytoplasm of the egg. |
巻・号 | 17(18) |
ページ | 1545-54 |
公開日 | 2007-9-18 |
DOI | 10.1016/j.cub.2007.08.049 |
PII | S0960-9822(07)01905-7 |
PMID | 17869113 |
MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Amino Acid Sequence Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology* Gene Expression Regulation, Developmental Green Fluorescent Proteins / analysis Meiosis Molecular Sequence Data Oocytes / enzymology* Oocytes / growth & development Phosphorylation Protein Tyrosine Phosphatases / chemistry Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / physiology* Protein-Tyrosine Kinases / analysis Protein-Tyrosine Kinases / metabolism* Recombinant Fusion Proteins / analysis Ubiquitin-Protein Ligases / metabolism Zygote / enzymology Zygote / growth & development Zygote / metabolism |
IF | 9.601 |
引用数 | 44 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
線虫 | tm1191 |