論文 - 詳細
| RRC ID | 3646 |
|---|---|
| 著者 | Whitten SJ, Miller MA. |
| タイトル | The role of gap junctions in Caenorhabditis elegans oocyte maturation and fertilization. |
| ジャーナル | Dev Biol |
| Abstract |
We have investigated the role of gap junctions in Caenorhabditis elegans oocyte maturation and fertilization. Gap junctions are observed between oocytes and the surrounding ovarian sheath cells in wild-type gonads. The sheath transcription factor CEH-18 is required to negatively regulate oocyte maturation, mitogen-activated protein kinase (MAPK) activation, and ovulation. Transmission electron microscopy (TEM) indicates that sheath/oocyte gap junctions are rare or absent in ceh-18(mg57) null mutant gonads. To test the hypothesis that gap junctions negatively regulate oocyte maturation, we performed an RNAi screen of innexin genes, which encode channel-forming proteins. Here we show that INX-14 and INX-22 are required in the female germ line to inhibit oocyte maturation, MAPK activation, and ovulation. Genetic analysis and TEM are consistent with INX-14 and INX-22 being components of sheath/oocyte gap junctions. Our results support the hypothesis that gap junctions maintain oocytes in meiotic prophase I when sperm are absent. We also implicate these channels in regulating sheath cell contractile activity and sperm recruitment to the spermatheca, the site of sperm storage and fertilization. Together with previous studies, our results help establish the C. elegans gonad as a model system for investigating the molecular mechanism(s) by which gap junctions regulate meiosis and fertilization. |
| 巻・号 | 301(2) |
| ページ | 432-46 |
| 公開日 | 2007-1-15 |
| DOI | 10.1016/j.ydbio.2006.08.038 |
| PII | S0012-1606(06)01108-0 |
| PMID | 16982048 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Connexins / genetics Enzyme Activation Female Fertilization / physiology* Gap Junctions / genetics Gap Junctions / metabolism* Gene Expression Regulation, Developmental Genome, Helminth / genetics Male Microscopy, Electron, Transmission Mitogen-Activated Protein Kinases / metabolism Mutation / genetics Oocytes / cytology* Oogenesis* RNA Interference Sperm Motility Spermatozoa / cytology Spermatozoa / physiology |
| IF | 2.896 |
| 引用数 | 46 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1661 |