論文 - 詳細
| RRC ID | 36760 |
|---|---|
| 著者 | Li C, Shi C, Kim J, Chen Y, Ni S, Jiang L, Zheng C, Li D, Hou J, Taichman RS, Sun H. |
| タイトル | Erythropoietin promotes bone formation through EphrinB2/EphB4 signaling. |
| ジャーナル | J Dent Res |
| Abstract |
Recent studies have demonstrated that erythropoietin (EPO) has extensive nonhematopoietic biological functions. However, little is known about how EPO regulates bone formation, although several studies suggested that EPO can affect bone homeostasis. In this study, we investigated the effects of EPO on the communication between osteoclasts and osteoblasts through the ephrinB2/EphB4 signaling pathway. We found that EPO slightly promotes osteoblastic differentiation with the increased expression of EphB4 in ST2 cells. However, EPO increased the expression of Nfatc1 and ephrinB2 but decreased the expression of Mmp9 in RAW264.7 cells, resulting in an increase of ephrinB2-expressing osteoclasts and a decrease in resorption activity. The stimulation of ephrinB2/EphB4 signaling via ephrinB2-Fc significantly promoted EPO-mediated osteoblastic differentiation in ST2 cells. EphB4 knockdown through EphB4 shRNA inhibited EPO-mediated osteoblastic phenotypes. Furthermore, in vivo assays clearly demonstrated that EPO efficiently induces new bone formation in the alveolar bone regeneration model. Taken together, these results suggest that ephrinB2/EphB4 signaling may play an important role in EPO-mediated bone formation. |
| 巻・号 | 94(3) |
| ページ | 455-63 |
| 公開日 | 2015-3-1 |
| DOI | 10.1177/0022034514566431 |
| PII | 0022034514566431 |
| PMID | 25586589 |
| PMC | PMC4336159 |
| MeSH | Alveolar Process / drug effects Animals Bone Regeneration / drug effects Cell Culture Techniques Cell Differentiation / drug effects Cell Line Ephrin-B2 / drug effects* Erythropoietin / pharmacology* Erythropoietin / therapeutic use Gene Knockdown Techniques Gene Silencing Incisor / surgery Macrophages / drug effects Male Matrix Metalloproteinase 9 / drug effects Mesenchymal Stem Cells / drug effects Mice NFATC Transcription Factors / drug effects Osteoblasts / drug effects Osteoclasts / drug effects Osteogenesis / drug effects* RNA, Small Interfering / administration & dosage Random Allocation Rats Rats, Wistar Receptor, EphB4 / drug effects* Signal Transduction / drug effects Tooth Extraction Tooth Socket / drug effects |
| IF | 4.914 |
| 引用数 | 38 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |