| RRC ID |
36997
|
| 著者 |
Ito T, Kondoh Y, Yoshida K, Umezawa T, Shimizu T, Shinozaki K, Osada H.
|
| タイトル |
Novel Abscisic Acid Antagonists Identified with Chemical Array Screening.
|
| ジャーナル |
Chembiochem
|
| Abstract |
Abscisic acid (ABA) signaling is involved in multiple processes in plants, such as water stress control and seed dormancy. Major regulators of ABA signaling are the PYR/PYL/RCAR family receptor proteins, group A protein phosphatases 2C (PP2Cs), and subclass III of SNF1-related protein kinase 2 (SnRK2). Novel ABA agonists and antagonists to modulate the functions of these proteins would not only contribute to clarification of the signaling mechanisms but might also be used to improve crop yields. To obtain small molecules that interact with Arabidopsis ABA receptor PYR1, we screened 24 275 compounds from a chemical library at the RIKEN Natural Products Depository by using a chemical array platform. Subsequent SnRK2 and PP2C assays narrowed down the candidates to two molecules. One antagonized ABA in a competitive manner and inhibited the formation of the PYR1-ABA-PP2C ternary complex. These compounds might have potential as bioprobes to analyze ABA signaling.
|
| 巻・号 |
16(17)
|
| ページ |
2471-8
|
| 公開日 |
2015-11-1
|
| DOI |
10.1002/cbic.201500429
|
| PMID |
26493545
|
| MeSH |
Abscisic Acid / chemistry
Abscisic Acid / pharmacology
Arabidopsis / growth & development
Arabidopsis / metabolism
Arabidopsis Proteins / antagonists & inhibitors*
Arabidopsis Proteins / metabolism
Germination / drug effects
High-Throughput Screening Assays
Kinetics
Membrane Transport Proteins / metabolism
Phosphoprotein Phosphatases / antagonists & inhibitors
Phosphoprotein Phosphatases / metabolism
Protein Serine-Threonine Kinases / antagonists & inhibitors
Protein Serine-Threonine Kinases / metabolism
Pyrans / chemistry
Seeds / growth & development
Seeds / metabolism
Signal Transduction / drug effects
Small Molecule Libraries / chemistry
Small Molecule Libraries / pharmacology
|
| IF |
2.576
|
| 引用数 |
9
|
|
WOS 分野
|
CHEMISTRY, MEDICINAL
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
rpc00008 |
