RRC ID 3700
Author Ohtaka A, Okuzaki D, Nojima H.
Title Mug27 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation.
Journal J Cell Sci
Abstract Several meiosis-specific proteins of Schizosaccharomyces pombe play essential roles in meiotic progression. We report here that a novel meiosis-specific protein kinase, Mug27 (also known as Ppk35), is required for proper spore formation. This kinase is expressed by the mug27(+) gene, which is abruptly transcribed after horsetail movement. This transcription is maintained until the second meiotic division. Green fluorescent protein (GFP)-tagged Mug27 appears at the start of prometaphase I, localizes to the spindle pole body (SPB) and then translocates to the forespore membrane (FSM) at late anaphase II. In the mug27Delta strain, smaller spores are produced compared with those of the mug27(+) strain. Moreover, spore viability was reduced by half or more compared with that of the mug27(+) strain. The protein-kinase activity of Mug27 appears to be important for its function: the putative kinase-dead Mug27 mutant had similar phenotypes to mug27Delta. Our results here indicate that the Mug27 kinase localizes at the SPB and regulates FSM formation and sporulation.
Volume 121(Pt 9)
Pages 1547-58
Published 2008-5-1
DOI 10.1242/jcs.022830
PII jcs.022830
PMID 18411246
MeSH Anaphase Cell Membrane / enzymology Conserved Sequence Green Fluorescent Proteins / metabolism Meiosis* Models, Biological Mutation / genetics Phenotype Protein Kinases / metabolism Protein Serine-Threonine Kinases / metabolism* Protein Transport Recombinant Fusion Proteins / metabolism Schizosaccharomyces / cytology* Schizosaccharomyces / enzymology* Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / enzymology Spores, Fungal / cytology Spores, Fungal / enzymology* Spores, Fungal / physiology* Vacuoles / enzymology
IF 4.573
Times Cited 15
Yeast CD16-1 FY9907(TP4-5A) FY7051(JZ670)