論文 - 詳細
| RRC ID | 37028 |
|---|---|
| 著者 | Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Matsumoto T, Nakata K, Mogi M. |
| タイトル | Interleukin-1β-induced autophagy-related gene 5 regulates proliferation of embryonic stem cell-derived odontoblastic cells. |
| ジャーナル | PLoS One |
| Abstract |
We previously established a method for the differentiation of induced pluripotent stem cells and embryonic stem cells into α2 integrin-positive odontoblast-like cells. We also reported that Wnt5 in response to interleukin (IL)-1β induces matrix metalloproteinase (MMP)-3-regulated cell proliferation in these cells. Our findings suggest that MMP-3 plays a potentially unique physiological role in the generation of odontoblast-like cells under an inflammatory state. Here, we examined whether up-regulation of autophagy-related gene (Atg) 5 by IL-1β was mediated by Wnt5 signaling, thus leading to increased proliferation of odontoblast-like cells. IL-1β increased the mRNA and protein levels of Atg5, microtubule-associated protein 1 light chain (LC3, a mammalian homolog of yeast Atg8) and Atg12. Treatment with siRNAs against Atg5, but not LC3 and Atg12, suppressed the IL-1β-induced increase in MMP-3 expression and cell proliferation. Our siRNA analyses combined with western blot analysis revealed a unique sequential cascade involving Atg5, Wnt5a and MMP-3, which resulted in the potent increase in odontoblastic cell proliferation. These results demonstrate the unique involvement of Atg5 in IL-1β-induced proliferation of embryonic stem cell-derived odontoblast-like cells. |
| 巻・号 | 10(4) |
| ページ | e0124542 |
| 公開日 | 2015-1-1 |
| DOI | 10.1371/journal.pone.0124542 |
| PII | PONE-D-14-54544 |
| PMID | 25894570 |
| PMC | PMC4403923 |
| MeSH | Animals Autophagy-Related Protein 5 Cell Differentiation / drug effects Cell Line Cell Proliferation / drug effects Embryonic Stem Cells / cytology* Embryonic Stem Cells / drug effects* Embryonic Stem Cells / metabolism Gene Expression Regulation, Enzymologic / drug effects Gene Silencing Matrix Metalloproteinase 3 / metabolism Mice Microtubule-Associated Proteins / deficiency Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Odontoblasts / cytology* Proteins / genetics Proteins / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / genetics Rats Signal Transduction / drug effects Wnt Proteins / genetics Wnt Proteins / metabolism |
| IF | 2.74 |
| 引用数 | 7 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B6G-2(AES0003) |