RRC ID 3725
Author Yamamoto A, Kitamura K, Hihara D, Hirose Y, Katsuyama S, Hiraoka Y.
Title Spindle checkpoint activation at meiosis I advances anaphase II onset via meiosis-specific APC/C regulation.
Journal J. Cell Biol.
Abstract During mitosis, the spindle assembly checkpoint (SAC) inhibits the Cdc20-activated anaphase-promoting complex/cyclosome (APC/C(Cdc20)), which promotes protein degradation, and delays anaphase onset to ensure accurate chromosome segregation. However, the SAC function in meiotic anaphase regulation is poorly understood. Here, we examined the SAC function in fission yeast meiosis. As in mitosis, a SAC factor, Mad2, delayed anaphase onset via Slp1 (fission yeast Cdc20) when chromosomes attach to the spindle improperly. However, when the SAC delayed anaphase I, the interval between meiosis I and II shortened. Furthermore, anaphase onset was advanced and the SAC effect was reduced at meiosis II. The advancement of anaphase onset depended on a meiosis-specific, Cdc20-related factor, Fzr1/Mfr1, which contributed to anaphase cyclin decline and anaphase onset and was inefficiently inhibited by the SAC. Our findings show that impacts of SAC activation are not confined to a single division at meiosis due to meiosis-specific APC/C regulation, which has probably been evolved for execution of two meiotic divisions.
Volume 182(2)
Pages 277-88
Published 2008-7-28
DOI 10.1083/jcb.200802053
PII jcb.200802053
PMID 18644893
PMC PMC2483520
MeSH Anaphase / genetics* Anaphase-Promoting Complex-Cyclosome Cdc20 Proteins Cdh1 Proteins Cell Cycle Proteins / genetics Chromosome Segregation / genetics Gene Expression Regulation, Fungal / genetics Genes, cdc / physiology* Mad2 Proteins Meiosis / genetics* Nuclear Proteins / genetics Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Spindle Apparatus / genetics* Spindle Apparatus / metabolism Ubiquitin-Protein Ligase Complexes / genetics*
IF 8.784
Times Cited 16
Yeast FY13046 FY9987(GT260)