RRC ID 3727
Author Nakase Y, Nakamura-Kubo M, Ye Y, Hirata A, Shimoda C, Nakamura T.
Title Meiotic spindle pole bodies acquire the ability to assemble the spore plasma membrane by sequential recruitment of sporulation-specific components in fission yeast.
Journal Mol Biol Cell
Abstract The spindle pole body (SPB) of Schizosaccharomyces pombe is required for assembly of the forespore membrane (FSM) during meiosis. Before de novo biogenesis of the FSM, the meiotic SPB forms outer plaques, an event referred to as SPB modification. A constitutive SPB component, Spo15, plays an indispensable role in SPB modification and sporulation. Here, we analyzed two sporulation-specific genes, spo13(+) and spo2(+), which are not required for progression of meiotic nuclear divisions, but are essential for sporulation. Spo13 is a 16-kDa coiled-coil protein, and Spo2 is a 15-kDa nonconserved protein. Both Spo13 and Spo2 specifically associated with the meiotic SPB. The respective deletion mutants are viable, but defective in SPB modification and in the onset of FSM formation. Spo13 and Spo2 localized on the cytoplasmic side of the SPB in close contact with the nascent FSM. Localization of Spo13 to the SPB was dependent on Spo15 and Spo2; that of Spo2 depended only on Spo15, suggesting that their recruitment to the SPB is strictly controlled. Spo2 physically associated with both Spo15 and Spo13, but Spo13 and Spo15 did not interact directly. Taken together, these observations indicate that Spo2 is recruited to the SPB during meiosis and then assists in the localization of Spo13 to the outer surface of the SPB.
Volume 19(6)
Pages 2476-87
Published 2008-6-1
DOI 10.1091/mbc.e08-02-0118
PII E08-02-0118
PMID 18367542
PMC PMC2397300
MeSH Cell Membrane / ultrastructure Gene Expression Regulation, Fungal Meiosis* Models, Biological Mutation / genetics Protein Binding Protein Transport Schizosaccharomyces / cytology* Schizosaccharomyces / genetics Schizosaccharomyces / physiology* Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / metabolism* Spindle Apparatus / ultrastructure Spores, Fungal / cytology* Spores, Fungal / ultrastructure Transcription, Genetic
IF 3.791
Times Cited 25
Yeast FY7071(MK13-2BL) FY7111(MK18-2CL) FY7132(TN8) FY7816(TN29) FY7273(TN104) FY7051(JZ670) FY7476(AB4) FY7434(SI52) FY7813(YN12) FY12275(YN47) FY12276(YN48) FY12295(YN67) FY12296(YN68) FY12305(YN77) FY12275(YN471) FYP1248(pBR(leu1)) FYP410(pTN143)