論文 - 詳細
RRC ID | 3746 |
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著者 | Kagoshima H, Sawa H, Mitani S, Bürglin TR, Shigesada K, Kohara Y. |
タイトル | The C. elegans RUNX transcription factor RNT-1/MAB-2 is required for asymmetrical cell division of the T blast cell. |
ジャーナル | Dev Biol |
Abstract |
The RUNX genes encode conserved transcription factors, which play vital roles in the development of various animals and human diseases. Drosophila runt is a secondary pair-rule gene, which regulates embryo segmentation. Human RUNX1, previously known as AML1, is essential for hematopoiesis. C. elegans rnt-1 is co-orthologous to the human RUNX genes. We found that RNT-1Colon, two colonsGFP is expressed in the H0-2, V1-6, and T blast cells in the embryo, and predominantly in the seam cells during larval to adult stages. rnt-1 mutants exhibit a loss of polarity in the asymmetrical T cell division in hermaphrodites and abnormal ray morphology in the male tail. Genetic and molecular analysis revealed that rnt-1 is allelic to mab-2. Mutant analysis suggested that rnt-1/mab-2 is involved in regulating T blast cell polarity in cooperation with the Wnt signaling pathway. Expression studies of GFPColon, two colonsPOP-1 and TLP-1Colon, two colonsGFP reporters in rnt-1/mab-2 mutants indicated that this gene functions upstream of tlp-1 and downstream, or in parallel to, pop-1 in the genetic cascade that controls asymmetry of the T cell division. All our data suggest that RNT-1/MAB-2 functions with POP-1 to control the asymmetry of the T cell division. |
巻・号 | 287(2) |
ページ | 262-73 |
公開日 | 2005-11-15 |
DOI | 10.1016/j.ydbio.2005.08.034 |
PII | S0012-1606(05)00562-2 |
PMID | 16226243 |
MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Caenorhabditis elegans / embryology Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Division / physiology Cell Lineage Cell Polarity / physiology DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Disorders of Sex Development Gene Expression Regulation, Developmental High Mobility Group Proteins / genetics High Mobility Group Proteins / metabolism Larva Molecular Sequence Data Mutation Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Signal Transduction T-Lymphocytes / physiology* Tail / anatomy & histology Tail / embryology Tail / growth & development Transcription Factors / genetics Transcription Factors / metabolism* |
IF | 2.896 |
引用数 | 26 |
WOS 分野 | DEVELOPMENTAL BIOLOGY |
リソース情報 | |
線虫 | tm388 tm491 |