RRC ID 37733
著者 Ikezoe T, Takeuchi T, Yang J, Adachi Y, Nishioka C, Furihata M, Koeffler HP, Yokoyama A.
タイトル Analysis of Aurora B kinase in non-Hodgkin lymphoma.
ジャーナル Lab Invest
Abstract This study explored the levels of Aurora B, a key regulator of mitosis, in 71 lymph nodes and tumor specimens excised operatively from individuals with various types of non-Hodgkin lymphoma (NHLs). Immunohistochemical examination found that diffuse large B-cell lymphoma (10/21, 48%) and Burkitt lymphoma (BL) (6/7, 86%) cells highly (percentage of positive cells, >20%) expressed Aurora B in their nuclei. On the other hand, none of the low-grade B-cell lymphoma (n=20), except for one case of follicular lymphoma, highly expressed this protein kinase, suggesting that levels of Aurora B correlated with histological grade in B-cell NHLs (P<0.01). Exposure of BL/leukemia cells to AZD1152-HQPA in vitro, a selective inhibitor of Aurora B kinase, potently induced growth arrest and apoptosis in a caspase-dependent, as well as -independent manner. Moreover, AZD1152 synergistically enhanced the effects of vincristine (VCR) to induce growth arrest of these cells. Further experiments found that VCR increased levels of the p-Aurora B through the activation of c-Jun N-terminal kinase, which was blocked in the presence of AZD1152-HQPA.
巻・号 89(12)
ページ 1364-73
公開日 2009-12-1
DOI 10.1038/labinvest.2009.106
PII labinvest2009106
PMID 19823168
MeSH Adult Aged Aged, 80 and over Apoptosis / drug effects Aurora Kinase B Aurora Kinases Caspases / metabolism Cell Line, Tumor Cell Proliferation / drug effects Drug Synergism Enzyme Activation Humans JNK Mitogen-Activated Protein Kinases / metabolism Lymphoma, Non-Hodgkin / enzymology* Middle Aged Organophosphates / pharmacology Phosphorylation Protein Serine-Threonine Kinases / antagonists & inhibitors Protein Serine-Threonine Kinases / metabolism* Quinazolines / pharmacology Signal Transduction / drug effects Tubulin Modulators / pharmacology Vincristine / pharmacology Young Adult
IF 4.197
引用数 30
WOS 分野 PATHOLOGY MEDICINE, RESEARCH & EXPERIMENTAL
リソース情報
ヒト・動物細胞 DAUDI(RCB1640)