論文 - 詳細
| RRC ID | 37783 |
|---|---|
| 著者 | Sohn SH, Ko E, Oh BG, Kim SH, Kim Y, Shin M, Hong M, Bae H. |
| タイトル | Inhibition effects of Vitex rotundifolia on inflammatory gene expression in A549 human epithelial cells. |
| ジャーナル | Ann Allergy Asthma Immunol |
| Abstract |
BACKGROUND:Vitex rotundifolia has long been used in traditional medicine to treat asthma and other allergic diseases. OBJECTIVE:To evaluate the anti-inflammatory mechanisms of V rotundifolia in cultured A549 human alveolar epithelial cells. METHODS:In the present study, A549 cells were stimulated with tumor necrosis factor alpha, interleukin 4, and interleukin 1beta to induce expression of chemokines and adhesion molecules involved in eosinophil chemotaxis. The anti-inflammatory effects of V rotundifolia on stimulated A549 cells were then evaluated by analyzing eotaxin secretion and eosinophil migration. In addition, the effects of V rotundifolia on gene expression profiles in stimulated A549 cells were evaluated by oligonucleotide microarray and real-time reverse transcription-polymerase chain reaction (RTRP). RESULTS:The V rotundifolia-treated A549 cells had significantly suppressed eotaxin secretion and eosinophil migration in a dose-dependent manner. In addition, the results of the microarray analysis and RTRP revealed that inflammation-related genes and cell adhesion-related genes were down-regulated in V rotundifolia-treated A549 cells. Furthermore, several genes related to the mitogen-activated protein kinase pathway were down-regulated in V rotundifolia-treated A549 cells. CONCLUSIONS:The mechanism responsible for the effects of V rotundifolia on A549 cells is closely associated with regulation of the mitogen-activated protein kinase pathway. Thus, V rotundifolia may be useful in the treatment of asthma and other allergic diseases. |
| 巻・号 | 103(2) |
| ページ | 152-9 |
| 公開日 | 2009-8-1 |
| DOI | 10.1016/S1081-1206(10)60169-X |
| PII | S1081-1206(10)60169-X |
| PMID | 19739429 |
| MeSH | Cell Adhesion Molecules / genetics Cell Line Cell Movement / drug effects Chemokine CCL11 / metabolism Chemokines / genetics Culture Media, Conditioned / pharmacology Cytokines / genetics* Down-Regulation / genetics Drugs, Chinese Herbal / pharmacology* Eosinophils / cytology Eosinophils / drug effects Epithelial Cells / drug effects* Epithelial Cells / metabolism* Gene Expression Profiling Gene Expression Regulation / drug effects* Gene Expression Regulation / genetics Humans Inflammation Mediators / metabolism* Interleukin-1beta / pharmacology Interleukin-4 / pharmacology MAP Kinase Signaling System / genetics Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha / pharmacology Up-Regulation / genetics Vitex / chemistry* |
| IF | 4.969 |
| 引用数 | 22 |
| WOS 分野 | ALLERGY IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |