| RRC ID |
37795
|
| 著者 |
Murakami T, Kariu T, Takazaki S, Hattori S, Chijiwa T, Ohno M, Oda-Ueda N.
|
| タイトル |
Island specific expression of a novel [Lys(49)]phospholipase A(2) (BPIII) in Protobothrops flavoviridis venom in Amami-Oshima, Japan.
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| ジャーナル |
Toxicon
|
| Abstract |
In search of the transcripts expressed in Protobothrops flavoviridis venom gland, 466 expressed sequence tags (ESTs) were generated from the venom gland cDNA library of P. flavoviridis in Amami-Oshima, Japan. The sequencing of randomly selected cDNA clones followed by identification in similarity search against existing databases led to the finding of a novel lysine-49-phospholipase A(2) ([Lys(49)]PLA(2)) clone. It coded for one amino acid-substituted BPII homologue or two amino acids-substituted BPI homologue in which BPII and BPI are [Lys(49)]PLA(2)s contained in Amami-Oshima and Tokunoshima P. flavoviridis venoms. This isozyme, named BPIII, was isolated from Amami-Oshima P. flavoviridis venom. BPIII gave a specific [M+2H](2+) peak of m/z 736.3 on mass spectrometry (MS) analysis after S-carboxamidomethylation and trypsin digestion when compared with BPII. It became evident from MS analysis after S-carboxamidomethylation and trypsin digestion of the mixed protein peaks ranging from BPI to BPII obtained by fractionation on a carboxymethyl cellulose column of Amami-Oshima and Tokunoshima P. flavoviridis venoms that BPIII protein is contained in Amami-Oshima P. flavoviridis venom but not in Tokunoshima P. flavoviridis venom. It is for the first time that a protein present in Amami-Oshima P. flavoviridis venom is not found in Tokunoshima P. flavoviridis venom.
|
| 巻・号 |
54(4)
|
| ページ |
399-407
|
| 公開日 |
2009-9-15
|
| DOI |
10.1016/j.toxicon.2009.05.003
|
| PII |
S0041-0101(09)00248-7
|
| PMID |
19463843
|
| MeSH |
Amino Acid Sequence
Amino Acid Substitution
Animals
Anticoagulants / chemistry
Anticoagulants / isolation & purification
Anticoagulants / metabolism*
Base Sequence
Chemical Fractionation
Crotalid Venoms / chemistry
Crotalid Venoms / enzymology*
Expressed Sequence Tags
Gene Library
Geography
Humans
Japan
Jurkat Cells
Lysine / chemistry
Mass Spectrometry
Molecular Sequence Data
Phospholipases A2 / chemistry
Phospholipases A2 / isolation & purification
Phospholipases A2 / metabolism*
Sequence Alignment
Viperidae / metabolism*
|
| IF |
2.201
|
| 引用数 |
7
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
TOXICOLOGY
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| リソース情報 |
| ヒト・動物細胞 |
|
