論文 - 詳細
| RRC ID | 3799 |
|---|---|
| 著者 | Ródenas E, Klerkx EP, Ayuso C, Audhya A, Askjaer P. |
| タイトル | Early embryonic requirement for nucleoporin Nup35/NPP-19 in nuclear assembly. |
| ジャーナル | Dev Biol |
| Abstract |
Nuclear pore complexes (NPCs) are gateways for transport between the nucleus and cytoplasm of eukaryotic cells and play crucial roles in regulation of gene expression. NPCs are composed of multiple copies of approximately 30 different nucleoporins (nups) that display both ubiquitous and cell type specific functions during development. Vertebrate Nup35 (also known as Nup53) was previously described to interact with Nup93, Nup155 and Nup205 and to be required for nuclear envelope (NE) assembly in vitro. Here, we report the first in vivo characterization of a Nup35 mutation, npp-19(tm2886), and its temperature-dependent effects on Caenorhabditis elegans embryogenesis. At restrictive temperature, npp-19(tm2886) embryos exhibit chromosome missegregation, nuclear morphology defects and die around mid-gastrulation. Depletion of Nup35/NPP-19 inhibits NE localization of Nup155/NPP-8, NPC assembly and nuclear lamina formation. Consequently, nuclear envelope function, including nucleo-cytoplasmic transport, is impaired. In contrast, recruitment of Nup107/NPP-5, LEM-2 and nuclear membranes to the chromatin surface is Nup35/NPP-19-independent, suggesting an uncoupling of nuclear membrane targeting and NPC assembly in the absence of Nup35/NPP-19. We propose that Nup35/NPP-19 has an evolutionary conserved role in NE formation and function, and that this role is particularly critical during the rapid cell divisions of early embryogenesis. |
| 巻・号 | 327(2) |
| ページ | 399-409 |
| 公開日 | 2009-3-15 |
| DOI | 10.1016/j.ydbio.2008.12.024 |
| PII | S0012-1606(08)01461-9 |
| PMID | 19146848 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Division / physiology Mutation Nuclear Envelope / metabolism* Nuclear Envelope / ultrastructure Nuclear Pore / metabolism* Nuclear Pore Complex Proteins / genetics Nuclear Pore Complex Proteins / metabolism* Protein Isoforms / genetics Protein Isoforms / metabolism* RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Temperature |
| IF | 2.896 |
| 引用数 | 24 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2886 |