RRC ID 380
著者 Miyashita T, Yeo SY, Hirate Y, Segawa H, Wada H, Little MH, Yamada T, Takahashi N, Okamoto H.
タイトル PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching.
ジャーナル Development
Abstract Slit is a secreted protein known to repulse the growth cones of commissural neurons. By contrast, Slit also promotes elongation and branching of axons of sensory neurons. The reason why different neurons respond to Slit in different ways is largely unknown. Islet2 is a LIM/homeodomain-type transcription factor that specifically regulates elongation and branching of the peripheral axons of the primary sensory neurons in zebrafish embryos. We found that PlexinA4, a transmembrane protein known to be a co-receptor for class III semaphorins, acts downstream of Islet2 to promote branching of the peripheral axons of the primary sensory neurons. Intriguingly, repression of PlexinA4 function by injection of the antisense morpholino oligonucleotide specific to PlexinA4 or by overexpression of the dominant-negative variant of PlexinA4 counteracted the effects of overexpression of Slit2 to induce branching of the peripheral axons of the primary sensory neurons in zebrafish embryos, suggesting involvement of PlexinA4 in the Slit signaling cascades for promotion of axonal branching of the sensory neurons. Colocalized expression of Robo, a receptor for Slit2, and PlexinA4 is observed not only in the primary sensory neurons of zebrafish embryos but also in the dendrites of the pyramidal neurons of the cortex of the mammals, and may be important for promoting the branching of either axons or dendrites in response to Slit, as opposed to the growth cone collapse.
巻・号 131(15)
ページ 3705-15
公開日 2004-8-1
DOI 10.1242/dev.01228
PII dev.01228
PMID 15229183
MeSH Animals Animals, Genetically Modified Axons / metabolism* Brain / cytology Brain / metabolism Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* In Situ Hybridization Intercellular Signaling Peptides and Proteins LIM-Homeodomain Proteins Molecular Sequence Data Nerve Tissue Proteins / classification Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Neurons, Afferent / cytology Neurons, Afferent / metabolism* Oligonucleotides, Antisense / genetics Oligonucleotides, Antisense / metabolism Phylogeny Receptors, Cell Surface / classification Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Signal Transduction / physiology* Transcription Factors / genetics Transcription Factors / metabolism* Zebrafish / anatomy & histology Zebrafish / embryology* Zebrafish Proteins*
IF 5.611
引用数 37
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ゼブラフィッシュ rw015d rw016a rw016b