論文 - 詳細
RRC ID | 38272 |
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著者 | Nakashima S, Matsuda H, Oda Y, Nakamura S, Xu F, Yoshikawa M. |
タイトル | Melanogenesis inhibitors from the desert plant Anastatica hierochuntica in B16 melanoma cells. |
ジャーナル | Bioorg Med Chem |
Abstract |
The methanolic extract from the whole plants of Anastatica hierochuntica, an Egyptian herbal medicine, was found to inhibit melanogenesis in theophylline-stimulated murine B16 melanoma 4A5 cells. Among the constituents isolated, anastatin A, silybin A, isosilybins A and B, eriodictyol, luteolin, kaempferol, quercetin, hierochins A and B, (2R,3S)-2,3-dihydro-2-(3,4-dimethoxyphenyl)-3-hydroxymethyl-5-(2-formylvinyl)-7-hydroxybenzofuran, (+)-dehydrodiconiferyl alcohol, (+)-balanophonin, 1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-hydroxy-1-propenyl]-2-methoxyphenoxy}-1,3-propanediol, and 3,4-dihydroxybenzaldehyde substantially inhibited melanogenesis with IC(50) values of 6.1-32 microM. With regard to the mechanism of action of silybins and isosilybins, the inhibition of tyrosinase activity suggested to be important. In addition, isosilybins A and B inhibited the mRNA expression of TRP-2, but silybins A and B oppositely enhanced the mRNA expression of tyrosinase and TRP-1 and -2 at 10 and/or 30 microM, and the inhibition of phosphorylation of extracellular signal-regulated kinases (ERK1/2) is involved in the enhanced expression of mRNA, at least in part, similar to that of PD98059. |
巻・号 | 18(6) |
ページ | 2337-2345 |
公開日 | 2010-3-15 |
DOI | 10.1016/j.bmc.2010.01.046 |
PII | S0968-0896(10)00067-2 |
PMID | 20189399 |
MeSH | Agaricales / enzymology Animals Antineoplastic Agents / chemistry Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology* Brassicaceae / chemistry* Cell Proliferation / drug effects Cell Survival / drug effects Drug Screening Assays, Antitumor Enzyme Inhibitors / chemistry Enzyme Inhibitors / isolation & purification Enzyme Inhibitors / pharmacology* Intramolecular Oxidoreductases / antagonists & inhibitors Intramolecular Oxidoreductases / genetics Intramolecular Oxidoreductases / metabolism Melanoma, Experimental / drug therapy* Melanoma, Experimental / genetics Melanoma, Experimental / pathology* Membrane Glycoproteins / antagonists & inhibitors Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism Mice Monophenol Monooxygenase / antagonists & inhibitors Monophenol Monooxygenase / genetics Monophenol Monooxygenase / metabolism Oxidoreductases / antagonists & inhibitors Oxidoreductases / genetics Oxidoreductases / metabolism Plant Extracts / chemistry Plant Extracts / isolation & purification Plant Extracts / pharmacology* RNA, Messenger / antagonists & inhibitors RNA, Messenger / genetics RNA, Messenger / metabolism Structure-Activity Relationship Tumor Cells, Cultured |
IF | 3.073 |
引用数 | 61 |
WOS 分野 | CHEMISTRY, MEDICINAL CHEMISTRY, ORGANIC BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | B16 melanoma 4A5(RCB0557) |