RRC ID 38356
Author Tokunaga R, Sakamoto Y, Nakagawa S, Miyake K, Izumi D, Kosumi K, Taki K, Higashi T, Imamura Y, Ishimoto T, Iwatsuki M, Baba Y, Miyamoto Y, Yoshida N, Oki E, Watanabe M, Baba H.
Title The Prognostic Significance of Histone Lysine Demethylase JMJD3/KDM6B in Colorectal Cancer.
Journal Ann. Surg. Oncol.
Abstract BACKGROUND:Jumonji-domain containing 3 (JMJD3) affects transcriptional regulation by demethylating lysine 27 residue of histone 3. We investigated its function and prognostic significance in colorectal cancer (CRC).
METHODS:The influence of JMJD3 on cell proliferation was assessed using quantitative RT-PCR and western blot on the downstream target gene of JMJD3, in knock-down (KD) experiments and clinical samples from 151 CRC patients.
RESULTS:Cells with KD JMJD3 significantly increased proliferation through cell cycle progression and apoptosis suppression. Expression of P15INK4B was remarkably decreased in KD JMJD3 cells; and JMJD3 expression strongly correlated with p15INK4B expression in clinical CRC samples (P < 0.001, r = 0.566). Low JMJD3 also was an independent predictor of poor prognosis (P = 0.042) in surgically resected CRC patients.
CONCLUSIONS:JMJD3 has prognostic significance in CRC and mediates p15INK4B expression. These results imply that elucidation of the JMJD3 role may lead to a new therapeutic approach for CRC patients.
Volume 23(2)
Pages 678-85
Published 2016-2
DOI 10.1245/s10434-015-4879-3
PII 10.1245/s10434-015-4879-3
PMID 26416711
MeSH Adult Aged Aged, 80 and over Apoptosis Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism* Cell Cycle Cell Proliferation Colorectal Neoplasms / enzymology Colorectal Neoplasms / genetics Colorectal Neoplasms / pathology* Female Follow-Up Studies Humans Immunoenzyme Techniques Jumonji Domain-Containing Histone Demethylases / genetics Jumonji Domain-Containing Histone Demethylases / metabolism* Male Middle Aged Neoplasm Invasiveness Neoplasm Staging Prognosis RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction Survival Rate Tumor Cells, Cultured
IF 3.681
Times Cited 4
Human and Animal Cells