Reference - Detail
|Author||Liang XT, Pan K, Chen MS, Li JJ, Wang H, Zhao JJ, Sun JC, Chen YB, Ma HQ, Wang QJ, Xia JC.|
|Title||Decreased expression of XPO4 is associated with poor prognosis in hepatocellular carcinoma.|
|Journal||J. Gastroenterol. Hepatol.|
BACKGROUND AND AIM:Exportin 4 (XPO4) is a recently-discovered candidate tumor-suppressor gene identified in a liver cancer mouse model. To investigate the role of XPO4 in hepatocellular carcinoma (HCC) pathogenesis, we determined XPO4 expression and its correlation to prognosis in human primary HCC.
METHODS:The XPO4 mRNA transcription level in HCC cell lines and tissue samples were detected by real-time quantitative polymerase chain reaction (PCR). XPO4 protein expression in 123 primary HCC clinical surgical specimens were analyzed by immunohistochemical detection.
RESULTS:Real-time quantitative PCR showed a decrease in XPO4 expression in HCC cell lines BEL-7402, Hep-G2, and SK-hep1 compared to the normal liver cell line LO2. Decreased XPO4 mRNA was also found in the majority of tumor tissues compared with matched non-tumor liver tissues (P = 0.004). Immunohistochemical detection revealed that XPO4 expression was reduced in 51 of 123 (41.5%) tumor resection samples compared with adjunct non-tumor tissues. We also found XPO4 expression to be significantly correlated with tumor size (P = 0.045) and histopathological classification (P = 0.004). Kaplan-Meier survival curves showed that the downregulation of XPO4 resulted in a significantly poor prognosis (P = 0.008, log-rank test), and multivariate Cox's analysis showed that XPO4 expression was an independent prognostic factor for overall survival of HCC patients (P = 0.013).
CONCLUSIONS:Our data suggest that XPO4 could be involved in the progression of human HCC and could serve as a potential target for gene therapy in the treatment of HCC.
|MeSH||Adult Aged Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism* Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / metabolism* Carcinoma, Hepatocellular / mortality Carcinoma, Hepatocellular / pathology Chi-Square Distribution China Down-Regulation Female Hep G2 Cells Humans Immunohistochemistry Kaplan-Meier Estimate Karyopherins / genetics Karyopherins / metabolism* Liver Neoplasms / genetics Liver Neoplasms / metabolism* Liver Neoplasms / mortality Liver Neoplasms / pathology Male Middle Aged Neoplasm Staging Prognosis Proportional Hazards Models RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Risk Assessment Risk Factors Survival Rate Time Factors Young Adult|
|WOS Category||GASTROENTEROLOGY & HEPATOLOGY|
|Human and Animal Cells|