| RRC ID |
38526
|
| 著者 |
Horie M, Nishio K, Endoh S, Kato H, Fujita K, Miyauchi A, Nakamura A, Kinugasa S, Yamamoto K, Niki E, Yoshida Y, Iwahashi H.
|
| タイトル |
Chromium(III) oxide nanoparticles induced remarkable oxidative stress and apoptosis on culture cells.
|
| ジャーナル |
Environ Toxicol
|
| Abstract |
Chromium(III) oxide (Cr(2)O(3)) is used for industrial applications such as catalysts and pigments. In the classical form, namely the fine particle, Cr(2)O(3) is insoluble and chemically stable. It is classified as a low-toxicity chromium compound. Recently, industrial application of nanoparticles (a new form composed of small particles with a diameter of ≤100 nm, in at least one dimension) has been increasing. Cellular effects induced by Cr(2)O(3) nanoparticles are not known. To shed light upon this, the release of soluble chromium from Cr(2)O(3) nano- and fine-particles in culture medium was compared. Fine Cr(2)O(3) particles were insoluble in the culture medium; on the contrary, Cr(2)O(3) nanoparticles released soluble hexavalent chromium into the culture medium. Cr(2)O(3) nanoparticles showed severe cytotoxicity. The effect of Cr(2)O(3) nanoparticles on cell viability was higher than that of fine particles. Cr(2)O(3) nanoparticles showed cytotoxicity equal to that of hexavalent chromium (K(2)Cr(2)O(7)). Human lung carcinoma A549 cells and human keratinocyte HaCaT cells showed an increase in intracellular reactive oxygen species (ROS) level and activation of antioxidant defense systems on exposure to Cr(2)O(3) nanoparticles. Exposure of Cr(2)O(3) nanoparticles led to caspase-3 activation, showing that the decrease in cell viability by exposure to Cr(2)O(3) nanoparticles was caused by apoptosis. Cellular responses were stronger in the Cr(2)O(3) nanoparticles-exposed cells than in fine Cr(2)O(3) - and CrCl(3) -exposed cells. Cellular uptake of Cr(2)O(3) particles were observed in nano- and fine-particles. The cellular influence of the extracellular soluble trivalent chromium was lower than that of Cr(2)O(3) nanoparticles. Cr(2)O(3) nanoparticles showed cytotoxicity by hexavalent chromium released at outside and inside of cells. The cellular influences of Cr(2)O(3) nanoparticles matched those of hexavalent chromium. In conclusion, Cr(2)O(3) nanoparticles have a high cytotoxic potential.
|
| 巻・号 |
28(2)
|
| ページ |
61-75
|
| 公開日 |
2013-2-1
|
| DOI |
10.1002/tox.20695
|
| PMID |
21384495
|
| MeSH |
Apoptosis / drug effects*
Caspase 3 / metabolism
Cell Line, Tumor
Cell Survival / drug effects
Chromium / chemistry
Chromium Compounds / pharmacology*
Culture Media / chemistry
DNA Damage
Glutathione / analysis
Humans
Keratinocytes / drug effects
Nanoparticles*
Oxidative Stress / drug effects*
Particle Size
Reactive Oxygen Species / metabolism
|
| IF |
3.118
|
| 引用数 |
43
|
|
WOS 分野
|
WATER RESOURCES
TOXICOLOGY
ENVIRONMENTAL SCIENCES
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098) |
