| RRC ID |
38623
|
| 著者 |
Yoshie S, Ito J, Shirasawa S, Yokoyama T, Fujimura Y, Takeda K, Mizuguchi M, Matsumoto K, Tomotsune D, Sasaki K.
|
| タイトル |
Establishment of novel detection system for embryonic stem cell-derived hepatocyte-like cells based on nongenetic manipulation with indocyanine green.
|
| ジャーナル |
Tissue Eng Part C Methods
|
| Abstract |
Hepatocytes derived from embryonic stem cells (ESCs) are expected to be useful for basic research and clinical applications. However, in several studies, genetic methods used to detect and obtain them are difficult and pose major safety problems. Therefore, in this study, we established a novel detection system for hepatocytes by using indocyanine green (ICG), which is selectively taken up by hepatocytes, based on nongenetic manipulation. ICG has maximum light absorption near 780 nm, and it fluoresces between 800 and 900 nm. Making use of these properties, we developed flow cytometry equipped with an excitation lazer of 785 nm and specific bandpass filters and successfully detected ESC-derived ICG-positive cells that were periodic acid-Schiff positive and expressed hepatocyte phenotypic mRNAs. These results demonstrate that this detection system based on nongenetic manipulation with ICG will lead to isolate hepatocytes generated from ESCs and provide the appropriate levels of stability, quality, and safety required for cell source for cell-based therapy and pharmaceutical studies such as toxicology.
|
| 巻・号 |
18(1)
|
| ページ |
12-20
|
| 公開日 |
2012-1-1
|
| DOI |
10.1089/ten.TEC.2011.0179
|
| PMID |
21902618
|
| MeSH |
Animals
Cell Culture Techniques*
Cells, Cultured
Coloring Agents / pharmacology
Embryonic Stem Cells / cytology*
Flow Cytometry / methods
HEK293 Cells
Hep G2 Cells
Hepatocytes / cytology*
Humans
Indocyanine Green / pharmacology*
Mice
Periodic Acid / pharmacology
Phenotype
Rats
Rats, Wistar
|
| IF |
3.508
|
| 引用数 |
3
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CELL & TISSUE ENGINEERING
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2 |
