| RRC ID |
38675
|
| 著者 |
Fujimoto N, Zhang J, Iwata K, Shinya T, Okada Y, Hayakawa T.
|
| タイトル |
A one-step sandwich enzyme immunoassay for tissue inhibitor of metalloproteinases-2 using monoclonal antibodies.
|
| ジャーナル |
Clin Chim Acta
|
| Abstract |
A one-step sandwich enzyme immunoassay system was developed with a pair of monoclonal antibodies against two individual oligopeptides prepared from the amino acid sequence of the human tissue inhibitor of metalloproteinases-2 (TIMP-2). The assay system consisting of two simultaneous immunoreactions used a solid phase monoclonal antibody and a horse-radish peroxidase-labeled monoclonal antibody. The system detected a free form of TIMP-2 and that complexed with active forms of matrix metalloproteinases (MMPs) giving a different sensitivity for each MMP but not TIMP-2 complexed with the precursor of 72 kDa gelatinase/type IV collagenase (MMP-2). The sensitivity of the system was 1.6 microgram/l (16 pg/assay) and linearity was obtained between 6.3 and 50 micrograms/l (63-500 pg/assay). TIMP-2 levels in the sera of 20 patients with rheumatoid arthritis (68 +/- 25 micrograms/l, mean +/- S.D.) and 13 patients with hepatocellular carcinoma (76 +/- 46 micrograms/l) were significantly higher (P < 0.05) than those of 18 normal subjects (5.6 +/- 7.4 micrograms/l). In contrast, the levels in the sera of 10 patients with gastric cancer (45 +/- 18 micrograms/l) and 7 patients with cancer of the uterus (36 +/- 13 micrograms/l) were significantly lower (P < 0.05 or P < 0.01) than those of normal subjects. Immunoreactivity analyses suggested that the precursor of MMP-2 in normal sera exists in a complexed form with TIMP-2 by interacting with the C-terminal domain of TIMP-2.
|
| 巻・号 |
220(1)
|
| ページ |
31-45
|
| 公開日 |
1993-10-29
|
| DOI |
10.1016/0009-8981(93)90004-n
|
| PII |
0009-8981(93)90004-N
|
| PMID |
8287559
|
| MeSH |
Amino Acid Sequence
Animals
Antibodies, Monoclonal*
Cells, Cultured
Chromatography, Gel
Female
Humans
Immunoenzyme Techniques*
Metalloendopeptidases / antagonists & inhibitors*
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Placenta / chemistry
Pregnancy
Proteins / analysis*
Proteins / isolation & purification
Sensitivity and Specificity
Tissue Inhibitor of Metalloproteinase-2
|
| IF |
2.615
|
| 引用数 |
110
|
|
WOS 分野
|
MEDICAL LABORATORY TECHNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NB1RGB(RCB0222) |
