論文 - 詳細
| RRC ID | 38699 |
|---|---|
| 著者 | Hu ZQ, Asano K, Yamazaki T, Shimamura T. |
| タイトル | Effect of lipopolysaccharide on mouse mast cell induction by a splenic cell culture system. |
| ジャーナル | Infect Immun |
| Abstract |
We have previously reported a method of mast cell induction by long-term culture of mouse spleen cells without using exogenous mast cell growth factor (Z.-Q. Hu, T. Yoshida, and T. Shimamura, J. Immunol. Methods 149:173, 1992). Supernatants recovered from the long-term cultures contain endogenous interleukin 3 and soluble stem cell factor. These were assessed by the capacity of the recovered supernatants to foster the growth of a mast cell growth factor-dependent cell line and by neutralizing antibodies. Besides the soluble factors, cell-to-cell contacts mediated by membrane stem cell factor on splenic stromal cells and c-Kit receptors on mast cells also affect mast cell induction. Different lots of fetal calf serum (FCS) were examined to determine a possible trigger for cytokine production. FCS can be divided into mast cell-inducible and noninducible sera by this process. However, not all FCS lots contain mast cell growth factor. The mast cell-inducible lots contain lipopolysaccharide (LPS) confirmed by a Limulus assay. Polymyxin B can neutralize the mast cell induction activity. Non-mast cell-inducible FCS can be converted to inducible FCS by adding exogenous LPS. The results indicate that LPS as a trigger of cytokine production is responsible for mast cell induction. |
| 巻・号 | 62(9) |
| ページ | 3844-9 |
| 公開日 | 1994-9-1 |
| DOI | 10.1128/iai.62.9.3844-3849.1994 |
| PMID | 7520422 |
| PMC | PMC303039 |
| MeSH | Animals Cattle Cell Division Cells, Cultured Fetal Blood / physiology Hematopoietic Cell Growth Factors / physiology Interleukin-3 / physiology Lipopolysaccharides / pharmacology* Male Mast Cells / drug effects* Mast Cells / physiology Mice Mice, Inbred C3H Polymyxin B / pharmacology Stem Cell Factor |
| IF | 3.201 |
| 引用数 | 14 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | IC-2(RCB0102) |