RRC ID 38713
Author Matsumoto H, Shimura M, Omatsu T, Okaichi K, Majima H, Ohnishi T.
Title p53 proteins accumulated by heat stress associate with heat shock proteins HSP72/HSC73 in human glioblastoma cell lines.
Journal Cancer Lett
Abstract We investigated the accumulation of p53 proteins after heat stress and their association with HSP72/HSC73 using four human glioblastoma cell lines. Human glioblastoma cell lines U-87MG and A-172 exhibited no mutation in the region between the 2nd and 11th exons of the p53 gene, whereas A-7 and T98G had mutations in exon 5 and exon 7 of the p53 gene, respectively. In U-87MG and A-172, the levels of wild-type p53 protein were slightly increased by heat stress. Levels of mutant p53 protein were apparently increased by heat stress in A-7, but not in T98G. Furthermore, wild-type p53 proteins in both U-87MG and A-172 co-immunoprecipitated with anti-HSP72/HSC73 antibody and HSP72 and HSC73 in them co-immunoprecipitated with anti-p53 antibody as did the mutant p53 proteins. These findings suggest that p53 proteins accumulated by heat stress are associated with HSP72 and HSC73.
Volume 87(1)
Pages 39-46
Published 1994-11-25
DOI 10.1016/0304-3835(94)90407-3
PII 0304-3835(94)90407-3
PMID 7954368
MeSH Blotting, Western DNA, Neoplasm / analysis Exons Genes, p53 Glioblastoma / metabolism* Glioblastoma / pathology HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism* Hot Temperature* Humans Mutation Polymerase Chain Reaction Precipitin Tests Tumor Cells, Cultured Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism*
IF 7.36
Times Cited 89
Human and Animal Cells U-87 MG(RCB0419)