| RRC ID |
38713
|
| 著者 |
Matsumoto H, Shimura M, Omatsu T, Okaichi K, Majima H, Ohnishi T.
|
| タイトル |
p53 proteins accumulated by heat stress associate with heat shock proteins HSP72/HSC73 in human glioblastoma cell lines.
|
| ジャーナル |
Cancer Lett
|
| Abstract |
We investigated the accumulation of p53 proteins after heat stress and their association with HSP72/HSC73 using four human glioblastoma cell lines. Human glioblastoma cell lines U-87MG and A-172 exhibited no mutation in the region between the 2nd and 11th exons of the p53 gene, whereas A-7 and T98G had mutations in exon 5 and exon 7 of the p53 gene, respectively. In U-87MG and A-172, the levels of wild-type p53 protein were slightly increased by heat stress. Levels of mutant p53 protein were apparently increased by heat stress in A-7, but not in T98G. Furthermore, wild-type p53 proteins in both U-87MG and A-172 co-immunoprecipitated with anti-HSP72/HSC73 antibody and HSP72 and HSC73 in them co-immunoprecipitated with anti-p53 antibody as did the mutant p53 proteins. These findings suggest that p53 proteins accumulated by heat stress are associated with HSP72 and HSC73.
|
| 巻・号 |
87(1)
|
| ページ |
39-46
|
| 公開日 |
1994-11-25
|
| DOI |
10.1016/0304-3835(94)90407-3
|
| PII |
0304-3835(94)90407-3
|
| PMID |
7954368
|
| MeSH |
Blotting, Western
DNA, Neoplasm / analysis
Exons
Genes, p53
Glioblastoma / metabolism*
Glioblastoma / pathology
HSP70 Heat-Shock Proteins / genetics
HSP70 Heat-Shock Proteins / metabolism*
Hot Temperature*
Humans
Mutation
Polymerase Chain Reaction
Precipitin Tests
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Protein p53 / metabolism*
|
| IF |
7.36
|
| 引用数 |
89
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
U-87 MG(RCB0419) |
