論文 - 詳細
| RRC ID | 38713 |
|---|---|
| 著者 | Matsumoto H, Shimura M, Omatsu T, Okaichi K, Majima H, Ohnishi T. |
| タイトル | p53 proteins accumulated by heat stress associate with heat shock proteins HSP72/HSC73 in human glioblastoma cell lines. |
| ジャーナル | Cancer Lett |
| Abstract |
We investigated the accumulation of p53 proteins after heat stress and their association with HSP72/HSC73 using four human glioblastoma cell lines. Human glioblastoma cell lines U-87MG and A-172 exhibited no mutation in the region between the 2nd and 11th exons of the p53 gene, whereas A-7 and T98G had mutations in exon 5 and exon 7 of the p53 gene, respectively. In U-87MG and A-172, the levels of wild-type p53 protein were slightly increased by heat stress. Levels of mutant p53 protein were apparently increased by heat stress in A-7, but not in T98G. Furthermore, wild-type p53 proteins in both U-87MG and A-172 co-immunoprecipitated with anti-HSP72/HSC73 antibody and HSP72 and HSC73 in them co-immunoprecipitated with anti-p53 antibody as did the mutant p53 proteins. These findings suggest that p53 proteins accumulated by heat stress are associated with HSP72 and HSC73. |
| 巻・号 | 87(1) |
| ページ | 39-46 |
| 公開日 | 1994-11-25 |
| DOI | 10.1016/0304-3835(94)90407-3 |
| PII | 0304-3835(94)90407-3 |
| PMID | 7954368 |
| MeSH | Blotting, Western DNA, Neoplasm / analysis Exons Genes, p53 Glioblastoma / metabolism* Glioblastoma / pathology HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism* Hot Temperature* Humans Mutation Polymerase Chain Reaction Precipitin Tests Tumor Cells, Cultured Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism* |
| IF | 7.36 |
| 引用数 | 89 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | U-87 MG(RCB0419) |