論文 - 詳細
RRC ID | 38819 |
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著者 | Yamada T, Jiping J, Endo R, Gotoh M, Shimosato Y, Hirohashi S. |
タイトル | Molecular cloning of a cell-surface glycoprotein that can potentially discriminate mesothelium from epithelium: its identification as vascular cell adhesion molecule 1. |
ジャーナル | Br J Cancer |
Abstract |
It has long been a practical problem for surgical pathologists to distinguish mesothelium from epithelium in order to make a positive diagnosis of mesothelioma. In this study, we developed a new monoclonal antibody, designated MS-2761 (IgG1, k), against cultured non-neoplastic mesothelial cells. Immunohistochemistry and slot-blot analysis revealed that this monoclonal antibody reacted with 100% (12/12) of benign and malignant mesothelioma tissues and a mesothelioma cell line, but not with 99% (77/78) of epithelial tumour tissues and 97% (33/34) of epithelial tumour cell lines. A gene encoding the cell-surface antigen defined by this monoclonal antibody was isolated from a mesothelial cell cDNA library constructed with a mammalian cell expression vector through transfection of Cos-7 cells and immunoselection by panning. DNA sequencing and a database search revealed that the gene was identical to vascular cell adhesion molecule 1 (VCAM1, also referred to as INCAM110). The prominent VCAM1 transcript in mesothelium was 3.2 kb in size with seven Ig-like domains, in addition to a minor transcripts with six Ig-like domains. This monoclonal antibody potentially discriminates mesothelium from epithelium and may become a tool for differential diagnosis of mesothelioma. |
巻・号 | 71(3) |
ページ | 562-70 |
公開日 | 1995-3-1 |
DOI | 10.1038/bjc.1995.110 |
PMID | 7533516 |
PMC | PMC2033655 |
MeSH | Animals Antibodies, Monoclonal / biosynthesis Base Sequence Blotting, Northern Cell Adhesion Molecules / analysis Cell Adhesion Molecules / genetics* Cell Adhesion Molecules / immunology Cells, Cultured Cloning, Molecular DNA / analysis DNA / genetics Epithelium / chemistry Epithelium / immunology Epithelium / physiology Humans Immunohistochemistry Membrane Glycoproteins / analysis Membrane Glycoproteins / genetics* Membrane Glycoproteins / immunology Mesothelioma / diagnosis* Mesothelioma / genetics Mesothelioma / pathology Mice Mice, Inbred BALB C Molecular Sequence Data Neoplasms / diagnosis Neoplasms / genetics Neoplasms / pathology Pleural Neoplasms / chemistry Pleural Neoplasms / pathology Polymerase Chain Reaction Precipitin Tests RNA-Directed DNA Polymerase Vascular Cell Adhesion Molecule-1 |
IF | 5.791 |
引用数 | 16 |
WOS 分野 | ONCOLOGY |
リソース情報 | |
ヒト・動物細胞 | COS-7(RCB0539) |