| RRC ID |
38841
|
| 著者 |
Tamura G, Sakata K, Nishizuka S, Maesawa C, Suzuki Y, Iwaya T, Terashima M, Saito K, Satodate R.
|
| タイトル |
Inactivation of the E-cadherin gene in primary gastric carcinomas and gastric carcinoma cell lines.
|
| ジャーナル |
Jpn J Cancer Res
|
| Abstract |
We investigated the E (epithelial)-cadherin gene for mutations and loss of heterozygosity (LOH) in 24 primary gastric carcinomas (12 differentiated and 12 undifferentiated types, including 3 signet-ring cell carcinomas), as well as 4 gastric carcinoma cell lines of the undifferentiated type (MKN-45, GCIY, HGC-27 and GT3TKB). We utilized PCR-SSCP and RT-PCR followed by direct sequencing to detect gene mutations and skipped exons, and RT-PCR-SSCP to examine LOH. In primary carcinomas, gene mutations or skipped exons were detected in 4 of 9 (44%) undifferentiated carcinomas of the scattered type, including 2 signet-ring cell carcinomas, and in none of the 3 undifferentiated carcinomas of the adherent type and 12 differentiated carcinomas. Demonstrated mutations of the E-cadherin gene included an 18 bp deletion (codon 418-423) and a 3 bp deletion (codon 400, calcium-binding domain), both located in exon 9. Skipping of exon 9 with a 1 bp insertion at codon 337, and skipping of exon 8 with a 1 bp deletion at codon 336, also were detected. LOH was confirmed in all of the carcinomas in which gene mutations or skipped exons (3/3 informative cases) were demonstrated. The MKN-45 cell line exhibited an 18 bp deletion at the exon 6-intron 6 boundary with loss of the wild-type allele, and 2 of the remaining 3 cell lines (HGC-27 and GT3TKB) had lost expression without detectable structural alteration of the E-cadherin gene. These data provide support for classic two-hit inactivation of the E-cadherin gene in a high percentage of undifferentiated carcinomas of the scattered type.
|
| 巻・号 |
87(11)
|
| ページ |
1153-9
|
| 公開日 |
1996-11-1
|
| DOI |
10.1111/j.1349-7006.1996.tb03125.x
|
| PII |
S0910505097871025
|
| PMID |
9045944
|
| PMC |
PMC5921011
|
| MeSH |
Alleles
Base Sequence
Cadherins / biosynthesis*
Cadherins / genetics*
Carcinoma / genetics*
Carcinoma / metabolism*
Exons
Gene Deletion
Gene Expression Regulation, Neoplastic*
Heterozygote
Humans
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Single-Stranded Conformational
Stomach Neoplasms / genetics*
Stomach Neoplasms / metabolism*
Transcription, Genetic
Tumor Cells, Cultured
|
| 引用数 |
100
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
GCIY(RCB0555)
HGC-27(RCB0500)
MKN45(RCB1001) |
