RRC ID 38851
著者 Osaki M, Miyata H, Hayashi A, Gomyo Y, Tatebe S, Ito H.
タイトル Lack of rearranged Tpr-met mRNA expression in human gastric cancer cell lines and gastric mucosa and carcinoma.
ジャーナル Anticancer Res
Abstract The met protooncogene was activated by a rearrangement involving the fusion of tpr (1q25) and met (7q21-31) gene sequence in a human osteosarcoma cell line (HOS) incubated in vitro with N-methyl-N-nitro-N-nitrosoguanidine (MNNG). We examined the expression of tpr-met mRNA by means of the reverse transcription-nested polymerase chain reaction (RT-nested PCR) in human two gastric cell lines (MKN-1 and MKN-45), T-cell acute lymphocytic leukemia cell line (MOLT-4), and in gastric tissue samples including normal mucosa, intestinal metaplasia and carcinoma from three surgical specimens. A DNA fragment of 88-bp was amplified in MKN-1 and MOLT-4, 96-bp in MKN-45 and of 58-bp in all nine tissue samples including gastric carcinomas. The amplified DNA sequences were not homologous with the rearranged tpr-met gene. Our study indicated that rearranged tpr-met mRNA is not expressed either in human gastric carcinoma cell lines or in gastric mucosa and carcinoma.
巻・号 16(5A)
ページ 2881-4
公開日 1996-1-1
PMID 8917402
MeSH Adenocarcinoma / genetics Adenocarcinoma / metabolism Base Sequence Carcinoma, Papillary / genetics Carcinoma, Papillary / metabolism Carcinoma, Signet Ring Cell / genetics Carcinoma, Signet Ring Cell / metabolism Gastric Mucosa / metabolism* Humans Molecular Sequence Data Nuclear Pore Complex Proteins Polymerase Chain Reaction / methods Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins c-met RNA, Messenger / genetics RNA, Messenger / metabolism* RNA, Neoplasm / genetics RNA, Neoplasm / metabolism* Receptor Protein-Tyrosine Kinases / genetics Receptor Protein-Tyrosine Kinases / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism* Stomach Neoplasms / genetics* Stomach Neoplasms / metabolism Tumor Cells, Cultured
IF 1.994
引用数 3
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 MKN45(RCB1001)