RRC ID |
38851
|
著者 |
Osaki M, Miyata H, Hayashi A, Gomyo Y, Tatebe S, Ito H.
|
タイトル |
Lack of rearranged Tpr-met mRNA expression in human gastric cancer cell lines and gastric mucosa and carcinoma.
|
ジャーナル |
Anticancer Res
|
Abstract |
The met protooncogene was activated by a rearrangement involving the fusion of tpr (1q25) and met (7q21-31) gene sequence in a human osteosarcoma cell line (HOS) incubated in vitro with N-methyl-N-nitro-N-nitrosoguanidine (MNNG). We examined the expression of tpr-met mRNA by means of the reverse transcription-nested polymerase chain reaction (RT-nested PCR) in human two gastric cell lines (MKN-1 and MKN-45), T-cell acute lymphocytic leukemia cell line (MOLT-4), and in gastric tissue samples including normal mucosa, intestinal metaplasia and carcinoma from three surgical specimens. A DNA fragment of 88-bp was amplified in MKN-1 and MOLT-4, 96-bp in MKN-45 and of 58-bp in all nine tissue samples including gastric carcinomas. The amplified DNA sequences were not homologous with the rearranged tpr-met gene. Our study indicated that rearranged tpr-met mRNA is not expressed either in human gastric carcinoma cell lines or in gastric mucosa and carcinoma.
|
巻・号 |
16(5A)
|
ページ |
2881-4
|
公開日 |
1996-1-1
|
PMID |
8917402
|
MeSH |
Adenocarcinoma / genetics
Adenocarcinoma / metabolism
Base Sequence
Carcinoma, Papillary / genetics
Carcinoma, Papillary / metabolism
Carcinoma, Signet Ring Cell / genetics
Carcinoma, Signet Ring Cell / metabolism
Gastric Mucosa / metabolism*
Humans
Molecular Sequence Data
Nuclear Pore Complex Proteins
Polymerase Chain Reaction / methods
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-met
RNA, Messenger / genetics
RNA, Messenger / metabolism*
RNA, Neoplasm / genetics
RNA, Neoplasm / metabolism*
Receptor Protein-Tyrosine Kinases / genetics
Receptor Protein-Tyrosine Kinases / metabolism*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism*
Stomach Neoplasms / genetics*
Stomach Neoplasms / metabolism
Tumor Cells, Cultured
|
IF |
1.994
|
引用数 |
3
|
WOS 分野
|
ONCOLOGY
|
リソース情報 |
ヒト・動物細胞 |
MKN45(RCB1001) |