RRC ID 38885
著者 Miyadera H, Bungener LB, Kusano S, Yokoyama S, Tokunaga K, Hepkema BG.
タイトル Questionable expression of unstable DQ heterodimer containing HLA-DQA1*01:07.
ジャーナル Tissue Antigens
Abstract Human leukocyte antigens (HLA)-DQA1*01:07 was identified as an HLA-DQ blank specificity that segregated with the serological HLA-A2, -B7, -DR14, -DR52 haplotype, which carried DQB1*05:03. The blank specificity of DQA1*01:07-DQB1*05:03 may be because of lack of reactivity of available typing sera, or disruption of proper assembly of DQ heterodimer. The cDNA sequence of DQA1*01:07 is nearly identical to DQA1*01:04 except for a variant at position 304, which results in the replacement of an arginine with a cysteine at 79α. To determine whether the DQA1*01:07 product can be expressed on cell-surface, we co-expressed DQA1*01:07 with various DQB1*05 or *06 alleles in fibroblast cells. Cell-surface expression of DQ was detectable when DQA1*01:07 was co-expressed with DQB1*06:04 but undetectable with other DQB1*05 and DQB1*06 alleles, including DQB1*05:03, to which DQA1*01:07 was encoded in cis. These data suggest that DQA1*01:07 may act as a phenotypically null allele in the DQA1*01:07-DQB1*05:03 haplotype, while it can be expressed at a low level in the presences of certain DQB1*06 alleles, such as DQB1*06:04, in trans. Based on the null or low expression of DQA1*01:07 as shown in the previous and present studies, DQA1*01:07 has recently been renamed to DQA1*01:07Q, indicating its questionable expression.
巻・号 86(6)
ページ 413-8
公開日 2015-12-1
DOI 10.1111/tan.12686
PMID 26555242
MeSH Alleles Amino Acid Substitution Animals DNA, Complementary / genetics Dimerization Gene Expression HLA-DQ beta-Chains / chemistry* Haplotypes Histocompatibility Testing Humans Mice Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed NIH 3T3 Cells Phenotype Protein Stability Sequence Alignment Sequence Homology, Amino Acid Terminology as Topic Transduction, Genetic
IF 1.348
引用数 3
WOS 分野 PATHOLOGY IMMUNOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 NIH3T3