| RRC ID |
38896
|
| 著者 |
Goto K, Kimura T, Kitamura N, Semba S, Ohmiya Y, Aburatani S, Matsukura S, Tsuda M, Kurokawa T, Ping Gong J, Tanaka S, Yasuda K.
|
| タイトル |
Synthetic PAMPS gel activates BMP/Smad signaling pathway in ATDC5 cells, which plays a significant role in the gel-induced chondrogenic differentiation.
|
| ジャーナル |
J Biomed Mater Res A
|
| Abstract |
The purposes of this study were to identify signaling pathways that were specifically activated in ATDC5 cells cultured on poly (2-acrylamido-2-methylpropanesulfonic acid) (PAMPS) gel in insulin-free maintenance medium and to evaluate the significance of the determined signaling pathways in the chondrogenic differentiation induced by this gel. In this study, ATDC5 cells cultured on PAMPS gel using the maintenance medium without insulin (PAMPS Culture) were compared with cells cultured on polystyrene using the differentiation medium containing insulin (PS-I Culture). The microarray analysis, Western blot analysis, and real-time PCR analysis demonstrated that the TGF-β/BMP signaling pathway was significantly enhanced at Days 1, 2, and 3 in the PAMPS Culture when compared with the PS-I Culture. Inhibition of the BMP type-I receptor reduced the phosphorylation level of Smad1/5 and expression of type-2 collagen and aggrecan mRNA in the cells accompanied by a reduction in cell aggregation at Day 13 in the PAMPS Culture. The inhibition of the TGF-β/BMP signaling pathway significantly inhibited the chondrogenic differentiation induced by the PAMPS gel. The present study demonstrated that synthetic PAMPS gel activates the TGF-β/BMP/Smad signaling pathway in the ATDC5 cells in the absence of insulin, and that this activation plays a significant role in the chondrogenic differentiation induced by PAMPS gel. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 734-746, 2016.
|
| 巻・号 |
104(3)
|
| ページ |
734-746
|
| 公開日 |
2016-3-1
|
| DOI |
10.1002/jbm.a.35615
|
| PMID |
26566602
|
| MeSH |
Animals
Bone Morphogenetic Protein Receptors / metabolism
Bone Morphogenetic Proteins / genetics
Bone Morphogenetic Proteins / metabolism*
Cattle
Cell Differentiation / drug effects*
Cell Line
Chondrogenesis / drug effects*
Gels / pharmacology*
Gene Expression Profiling
Gene Expression Regulation / drug effects
Mice
Phosphorylation / drug effects
Polymers / pharmacology*
Polystyrenes / chemistry
RNA, Messenger / genetics
RNA, Messenger / metabolism
Signal Transduction / drug effects*
Smad Proteins / metabolism*
Sulfonic Acids / pharmacology*
Transforming Growth Factor beta / metabolism
|
| IF |
3.525
|
| 引用数 |
7
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
