論文 - 詳細
| RRC ID | 38998 |
|---|---|
| 著者 | Kawaguchi Y, Tanaka H, Okada T, Konishi H, Takahashi M, Ito M, Asai J. |
| タイトル | Effect of reactive oxygen species on the elastin mRNA expression in cultured human dermal fibroblasts. |
| ジャーナル | Free Radic Biol Med |
| Abstract |
The effects of ultraviolet A (UVA) and reactive oxygen species (ROS), generated by a xanthine and xanthine oxidase (XOD) system, on the mRNA expression of elastin, were investigated using cultured human dermal fibroblasts. Total RNA was isolated and subjected to Northern blot analysis using synthesized 530 base cDNA probe for elastin with primers derived from exon 10 and 1 of human elastin. UVA irradiation did not affect elastin mRNA expression. In contrast, ROS resulted in a dose-related increase in the level of elastin mRNA up to 1.8-fold in cultured human dermal fibroblasts. Catalase, used as scavenger, essentially prevented the ROS induced alterations in elastin mRNA levels. These results suggest that ROS produced in the dermis may contribute to elastin deposition observed in photoaging skin. |
| 巻・号 | 23(1) |
| ページ | 162-5 |
| 公開日 | 1997-1-1 |
| DOI | 10.1016/s0891-5849(96)00570-9 |
| PII | S0891-5849(96)00570-9 |
| PMID | 9165309 |
| MeSH | Blotting, Northern Catalase / pharmacology Cells, Cultured Elastin / genetics* Fibroblasts Gene Expression Regulation Humans RNA, Messenger / genetics RNA, Messenger / metabolism* Reactive Oxygen Species / metabolism* Skin / metabolism* Skin / radiation effects Skin Aging / genetics Skin Aging / physiology* Ultraviolet Rays Xanthine Xanthine Oxidase / metabolism Xanthines / metabolism |
| IF | 6.17 |
| 引用数 | 49 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |