論文 - 詳細
| RRC ID | 39027 |
|---|---|
| 著者 | Kanzaki M, Lindorfer MA, Garrison JC, Kojima I. |
| タイトル | Activation of the calcium-permeable cation channel CD20 by alpha subunits of the Gi protein. |
| ジャーナル | J Biol Chem |
| Abstract |
When the calcium-permeable cation channel CD20 is expressed in Balb/c 3T3 cells, it is activated by insulin-like growth factor-I (IGF-I) via the IGF-I receptor (Kanzaki, M., Nie, L., Shibata, H., and Kojima, I. (1997) J. Biol. Chem. 272, 4964-4969). The present study was conducted to investigate the role of G proteins in the regulation of the CD20 channel. In the excised patch clamp mode, activation of the CD20 channel by IGF-I required GTP, Mg2+, and ATP in the bath solution, and removal of either GTP or ATP attenuated the activation. Non-hydrolyzable ATP could substitute for ATP, and guanyl-5'-yl thiophosphate blocked the activation of the channel by IGF-I. The CD20 channel was also activated by guanosine 5'-3-O-(thio)triphosphate, and ATP was not required for the activation. Addition of a preparation of Gi/Go holoprotein purified from bovine brain activated the CD20, and the beta-adrenergic receptor kinase peptide did not affect the number of channel openings induced by the G protein. The CD20 channel was stimulated by the GTP-bound form of recombinant Gi2 alpha subunit purified from Sf9 cells. The Gi3 alpha subunit was less effective, and the Gi1 alpha subunit had no effect. Purified recombinant beta1gamma2 subunits did not affect the activity of the channel. Finally, IGF-I-induced activation of CD20 was inhibited by an antibody against Gi2 alpha subunit. These findings indicate that the CD20 channel expressed in Balb/c 3T3 cells is activated by the IGF-I receptor via the alpha subunits of heterotrimeric G proteins. |
| 巻・号 | 272(23) |
| ページ | 14733-9 |
| 公開日 | 1997-6-6 |
| DOI | 10.1074/jbc.272.23.14733 |
| PII | S0021-9258(19)62453-1 |
| PMID | 9169438 |
| MeSH | 3T3 Cells Adenosine Triphosphate / analogs & derivatives Adenosine Triphosphate / pharmacology Adenylyl Imidodiphosphate / pharmacology Animals Antigens, CD20 / drug effects Antigens, CD20 / physiology* Brain / metabolism Cattle GTP-Binding Proteins / chemistry GTP-Binding Proteins / isolation & purification GTP-Binding Proteins / metabolism* Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology Humans Insulin-Like Growth Factor I / pharmacology Kinetics Macromolecular Substances Membrane Potentials / drug effects Mice Mice, Inbred BALB C Patch-Clamp Techniques Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Transfection Virulence Factors, Bordetella / pharmacology |
| IF | 4.238 |
| 引用数 | 31 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BALB/3T3 clone A31(RCB0005) |