論文 - 詳細
| RRC ID | 39467 |
|---|---|
| 著者 | Ishida M, Ohbayashi N, Fukuda M. |
| タイトル | Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. |
| ジャーナル | Sci Rep |
| Abstract |
Melanosomes are lysosome-related organelles in melanocytes that are transported from the perinucleus to the cell periphery by coordination between bidirectional (anterograde and retrograde) microtubule-dependent transport and unidirectional actin-dependent transport. Although the molecular machineries that mediate retrograde transport and actin-dependent transport have already been identified, little is known about the anterograde transport complex on microtubules in mammalian cells. Here we discovered that small GTPase Rab1A on melanosomes recruits SKIP/PLEKHM2 as a Rab1A-specific effector and that Rab1A, SKIP, and a kinesin-1/(Kif5b+KLC2) motor form a transport complex that mediates anterograde melanosome transport in melanocytes. Interestingly, Arl8, Arf-like small GTPase that also interacts with SKIP, is specifically localized at lysosomes and regulates their anterograde transport in melanocytes. Our findings suggest that the anterograde microtubule-dependent transport of melanosomes and lysosomes are differently regulated by independent cargo receptors, i.e., Rab1A and Arl8, respectively, but that a SKIP-kinesin-1 mechanism is responsible for the transport of both. |
| 巻・号 | 5 |
| ページ | 8238 |
| 公開日 | 2015-2-4 |
| DOI | 10.1038/srep08238 |
| PII | srep08238 |
| PMID | 25649263 |
| PMC | PMC4316160 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism* Animals Biological Transport Gene Knockdown Techniques Humans Kinesins / chemistry Kinesins / genetics Kinesins / metabolism* Melanocytes / metabolism Melanosomes / metabolism* Multiprotein Complexes / metabolism Protein Binding Protein Interaction Domains and Motifs Protein Interaction Mapping Two-Hybrid System Techniques rab1 GTP-Binding Proteins / metabolism* |
| IF | 3.998 |
| 引用数 | 15 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pEGFP-C1-SKIP/PLEKHM2 (RDB13232) pmStr-C1-SKIP/PLEKHM2 (RDB13233) pmStr-N1-Arl8b (RDB13234) pmStr-N1-Arl8b (Q75L) (RDB13235) pmStr-N1-mouse Arl8b (RDB15388). |