Reference - Detail
| RRC ID | 39501 |
|---|---|
| Author | Nakatani T, Yamagata K, Kimura T, Oda M, Nakashima H, Hori M, Sekita Y, Arakawa T, Nakamura T, Nakano T. |
| Title | Stella preserves maternal chromosome integrity by inhibiting 5hmC-induced γH2AX accumulation. |
| Journal | EMBO Rep |
| Abstract |
In the mouse zygote, Stella/PGC7 protects 5-methylcytosine (5mC) of the maternal genome from Tet3-mediated oxidation to 5-hydroxymethylcytosine (5hmC). Although ablation of Stella causes early embryonic lethality, the underlying molecular mechanisms remain unknown. In this study, we report impaired DNA replication and abnormal chromosome segregation (ACS) of maternal chromosomes in Stella-null embryos. In addition, phosphorylation of H2AX (γH2AX), which has been reported to inhibit DNA replication, accumulates in the maternal chromatin of Stella-null zygotes in a Tet3-dependent manner. Cell culture assays verified that ectopic appearance of 5hmC induces abnormal accumulation of γH2AX and subsequent growth retardation. Thus, Stella protects maternal chromosomes from aberrant epigenetic modifications to ensure early embryogenesis. |
| Volume | 16(5) |
| Pages | 582-9 |
| Published | 2015-5-1 |
| DOI | 10.15252/embr.201439427 |
| PII | embr.201439427 |
| PMID | 25694116 |
| PMC | PMC4428038 |
| MeSH | 5-Methylcytosine / analogs & derivatives Animals Cell Division Cell Line Cells, Cultured Chromosomal Instability* Chromosomal Proteins, Non-Histone Chromosome Aberrations Chromosome Segregation Cytosine / analogs & derivatives* Cytosine / metabolism DNA Methylation DNA Replication DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Dioxygenases Embryo, Mammalian / cytology Embryo, Mammalian / metabolism Epigenesis, Genetic Female Gene Knockout Techniques Histones / metabolism* Male Mice Protein Transport Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism* Zygote / metabolism |
| IF | 7.497 |
| Times Cited | 22 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | CSII-EF-MCS-IRES2-Venus (RDB04384) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393). |