論文 - 詳細
| RRC ID | 39789 |
|---|---|
| 著者 | Kato K, Satouh Y, Nishimasu H, Kurabayashi A, Morita J, Fujihara Y, Oji A, Ishitani R, Ikawa M, Nureki O. |
| タイトル | Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization. |
| ジャーナル | Nat Commun |
| Abstract |
Sperm-egg fusion is the critical step in mammalian fertilization, and requires the interaction between IZUMO1 on the sperm surface and JUNO (also known as folate receptor (FR) 4 or IZUMO1R) on the egg surface. Whereas other FRs bind and uptake folates, JUNO binds IZUMO1 and establishes the cell-cell adhesion. However, the mechanism of IZUMO1 recognition by JUNO has remained elusive. Here we report the crystal structure of mouse JUNO, at 2.3 Å resolution. A structural comparison of JUNO with the FRs revealed that JUNO and the FRs have similar overall structures, but JUNO lacks the folate-binding pocket, thereby explaining the inability of JUNO to bind folate. Further complementation of Juno knockout eggs with mutant Juno messenger RNAs revealed that the conserved, surface-exposed tryptophan residue of JUNO is required for sperm binding and fertilization. Our structure-based in vivo functional analyses provide a framework towards a mechanistic understanding of mammalian gamete recognition. |
| 巻・号 | 7 |
| ページ | 12198 |
| 公開日 | 2016-7-15 |
| DOI | 10.1038/ncomms12198 |
| PII | ncomms12198 |
| PMID | 27416963 |
| PMC | PMC4947182 |
| MeSH | Animals Binding Sites Carrier Proteins / genetics Carrier Proteins / metabolism Cell Adhesion Crystallography, X-Ray Egg Proteins Female Fertilization / physiology* Folate Receptor 1 / chemistry Folate Receptor 1 / metabolism HEK293 Cells Humans Immunoglobulins / chemistry Immunoglobulins / genetics Immunoglobulins / metabolism* Male Membrane Proteins / chemistry Membrane Proteins / genetics Membrane Proteins / metabolism* Mice, Knockout Mutation Receptors, Cell Surface / chemistry* Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Sperm-Ovum Interactions / physiology Tryptophan / genetics Tryptophan / metabolism |
| IF | 12.121 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | EGR-G101(AES0182) 293T(RCB2202) |