RRC ID 398
Author Wada H, Iwasaki M, Sato T, Masai I, Nishiwaki Y, Tanaka H, Sato A, Nojima Y, Okamoto H.
Title Dual roles of zygotic and maternal Scribble1 in neural migration and convergent extension movements in zebrafish embryos.
Journal Development
Abstract In the developing vertebrate hindbrain, the characteristic trajectory of the facial (nVII) motor nerve is generated by caudal migration of the nVII motor neurons. The nVII motor neurons originate in rhombomere (r) 4, and migrate caudally into r6 to form the facial motor nucleus. In this study, using a transgenic zebrafish line that expresses green fluorescent protein (GFP) in the cranial motor neurons, we isolated two novel mutants, designated landlocked (llk) and off-road (ord), which both show highly specific defects in the caudal migration of the nVII motor neurons. We show that the landlocked locus contains the gene scribble1 (scrb1), and that its zygotic expression is required for migration of the nVII motor neurons mainly in a non cell-autonomous manner. Taking advantage of the viability of the llk mutant embryos, we found that maternal expression of scrb1 is required for convergent extension (CE) movements during gastrulation. Furthermore, we show a genetic interaction between scrb1 and trilobite(tri)/strabismus(stbm) in CE. The dual roles of the scrb1 gene in both neuronal migration and CE provide a novel insight into the underlying mechanisms of cell movement in vertebrate development.
Volume 132(10)
Pages 2273-85
Published 2005-5
DOI 10.1242/dev.01810
PII dev.01810
PMID 15829519
MeSH Animals Base Sequence Cell Movement / genetics Cell Movement / physiology* Chromosome Mapping Crosses, Genetic DNA Primers Facial Nerve / embryology* Gastrula / metabolism Gene Expression Regulation, Developmental* Green Fluorescent Proteins / metabolism Immunohistochemistry In Situ Hybridization Membrane Proteins / genetics* Membrane Proteins / metabolism* Molecular Sequence Data Motor Neurons / cytology* Mutation / genetics Oligonucleotides, Antisense Reverse Transcriptase Polymerase Chain Reaction Rhombencephalon / embryology Sequence Analysis, DNA Zebrafish / embryology* Zebrafish Proteins / genetics* Zebrafish Proteins / metabolism*
IF 5.413
Times Cited 84
Zebrafish rw16 RIKEN WT (RW)