| RRC ID |
39801
|
| 著者 |
Ohkawa K, Takada K, Asakura T, Hashizume Y, Okawa Y, Tashiro K, Ueda J, Itoh Y, Hibi N.
|
| タイトル |
Calpain inhibitor inhibits secretory granule maturation and secretion of GH.
|
| ジャーナル |
Neuroreport
|
| Abstract |
Clathrin- and AP-1-coated buds are present on immature secretory granules of endocrine cells that mature into clathrin-uncoated granules. The mechanism of clathrin and adaptor protein uncoating has remained obscure. Benzyloxycarbonyl-L-leucyl-L-leucinal (ZLLal), a calpain inhibitor, reduced growth hormone (GH) secretion with intracellular accumulation, in a GH-secreting rat pituitary tumor cell. Pulse and chase demonstrated that ZLLal retarded the turnover of clathrin (Clt.H) and adaptins. ZLLal-treatment co-immunoprecipitated the increased amounts of GH with Clt.H and adaptins compared to control cells, suggesting the intracellular accumulation of immature secretory granules. Clt.H and adaptins were limited-proteolyzed by m-calpain in vitro, indicating that calpain may be involved partly in the maturation of secretory granules in endocrine cells via the process of clathrin uncoating.
|
| 巻・号 |
11(18)
|
| ページ |
4007-11
|
| 公開日 |
2000-12-18
|
| DOI |
10.1097/00001756-200012180-00021
|
| PMID |
11192619
|
| MeSH |
Adaptor Protein Complex alpha Subunits
Adaptor Proteins, Vesicular Transport
Animals
Calpain / antagonists & inhibitors
Calpain / metabolism*
Clathrin / drug effects
Clathrin / metabolism*
Cysteine Proteinase Inhibitors / pharmacology
Dipeptides / pharmacology
Dose-Response Relationship, Drug
Glycoproteins / pharmacology*
Golgi Apparatus / drug effects
Golgi Apparatus / metabolism
Growth Hormone / metabolism*
Growth Hormone-Releasing Hormone / pharmacology
Membrane Proteins / drug effects
Membrane Proteins / metabolism*
Pituitary Gland, Anterior / drug effects
Pituitary Gland, Anterior / metabolism*
Pituitary Gland, Anterior / ultrastructure
Rats
Secretory Vesicles / drug effects
Secretory Vesicles / metabolism*
Tumor Cells, Cultured / drug effects
Tumor Cells, Cultured / metabolism
|
| IF |
1.394
|
| 引用数 |
12
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| ヒト・動物細胞 |
MtT/S(RCB0528) |
