論文 - 詳細
| RRC ID | 39822 |
|---|---|
| 著者 | Abe Y, Nakayama K, Yamanaka A, Sakurai T, Goto K. |
| タイトル | Subtype-specific trafficking of endothelin receptors. |
| ジャーナル | J Biol Chem |
| Abstract |
We investigated the subcellular localization of two endothelin receptors (ET(A)R and ET(B)R). To visualize these receptors directly, the C terminus of each receptor was fused to the N terminus of enhanced green fluorescent protein (designated as ETR-EGFP). When transiently expressed in various mammalian cell lines, ET(A)R-EGFP was predominantly localized on the plasma membrane. By contrast, ET(B)R-EGFP was, independent of ligand stimulation, predominantly localized on the intracellular vesicular structures containing Lamp-1. Immunoblot analyses revealed that at steady state ET(B)R-EGFP was highly degraded, and its degradation was inhibited by bafilomycin A(1). Antibody uptake experiments suggested that the ET(B)R-EGFP molecules were internalized from the plasma membrane. It is therefore likely that ET(B)R is first transported to the plasma membrane and then internalized, irrespective of ligand stimulation, to lysosomes where it undergoes proteolytic degradation. Exchanging the C-terminal cytoplasmic tails of the two ETRs revealed that the cytoplasmic tail is responsible for both the intracellular localization and the degradation of the receptors. Deletion of the extreme C-terminal 35 amino acids from both receptors allowed the receptor proteins to localize predominantly in the intracellular vesicles and to degrade. These observations indicate that the cytoplasmic tail of ET(A)R determines its plasma membrane localization. Stimulation with endothelin-1 increased the amount of intact ETR-EGFP fusion proteins without increasing their de novo synthesis, suggesting that binding of endothelin-1 stabilizes the ETRs. |
| 巻・号 | 275(12) |
| ページ | 8664-71 |
| 公開日 | 2000-3-24 |
| DOI | 10.1074/jbc.275.12.8664 |
| PII | S0021-9258(18)30173-X |
| PMID | 10722707 |
| MeSH | Amino Acid Sequence Animals Antigens, CD / isolation & purification Biological Transport Cell Compartmentation Cell Membrane / metabolism Endocytosis Endothelin-1 / metabolism Endothelin-3 / metabolism Green Fluorescent Proteins Ligands Luminescent Proteins / genetics Luminescent Proteins / metabolism Lysosome-Associated Membrane Glycoproteins Lysosomes / metabolism Membrane Glycoproteins / isolation & purification Models, Molecular Molecular Sequence Data Peptide Fragments / genetics Peptide Fragments / metabolism Rats Receptor, Endothelin A Receptor, Endothelin B Receptors, Endothelin / genetics Receptors, Endothelin / metabolism* Recombinant Fusion Proteins / metabolism Sequence Homology, Amino Acid Signal Transduction |
| IF | 4.238 |
| 引用数 | 47 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LTK-(RCB0208) |