RRC ID |
3993
|
著者 |
Cartagena JA, Matsunaga S, Seki M, Kurihara D, Yokoyama M, Shinozaki K, Fujimoto S, Azumi Y, Uchiyama S, Fukui K.
|
タイトル |
The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollen.
|
ジャーナル |
Dev Biol
|
Abstract |
Plant SET domain proteins are known to be involved in the epigenetic control of gene expression during plant development. Here, we report that the Arabidopsis SET domain protein, SDG4, contributes to the epigenetic regulation of pollen tube growth, thus affecting fertilization. Using an SDG4-GFP fusion construct, the chromosomal localization of SDG4 was established in tobacco BY-2 cells. In Arabidopsis, sdg4 knockout showed reproductive defects. Tissue-specific expression analyses indicated that SDG4 is the major ASH1-related gene expressed in the pollen. Immunological analyses demonstrated that SDG4 was involved in the methylation of histone H3 in the inflorescence and pollen grains. The significant reduction in the amount of methylated histone H3 K4 and K36 in sdg4 pollen vegetative nuclei resulted in suppression of pollen tube growth. Our results indicate that SDG4 is capable of modulating the expression of genes that function in the growth of pollen tube by methylation of specific lysine residues of the histone H3 in the vegetative nuclei.
|
巻・号 |
315(2)
|
ページ |
355-68
|
公開日 |
2008-3-15
|
DOI |
10.1016/j.ydbio.2007.12.016
|
PII |
S0012-1606(07)01602-8
|
PMID |
18252252
|
MeSH |
Amino Acid Sequence
Arabidopsis / genetics
Arabidopsis / growth & development*
Arabidopsis / metabolism*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Base Sequence
Cell Line
DNA Primers / genetics
DNA, Plant / genetics
Genes, Plant
Histone-Lysine N-Methyltransferase / genetics
Histone-Lysine N-Methyltransferase / metabolism
Histones / chemistry
Histones / genetics
Histones / metabolism*
Lysine / chemistry
Lysine / metabolism
Methylation
Molecular Sequence Data
Mutation
Oligonucleotide Array Sequence Analysis
Plants, Genetically Modified
Pollen / genetics
Pollen / metabolism
Pollen Tube / growth & development*
Pollen Tube / metabolism*
Sequence Homology, Amino Acid
Tobacco
|
IF |
2.896
|
引用数 |
65
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda03998 |