論文 - 詳細
| RRC ID | 39978 |
|---|---|
| 著者 | Randlett O, Wee CL, Naumann EA, Nnaemeka O, Schoppik D, Fitzgerald JE, Portugues R, Lacoste AM, Riegler C, Engert F, Schier AF. |
| タイトル | Whole-brain activity mapping onto a zebrafish brain atlas. |
| ジャーナル | Nat Methods |
| Abstract |
In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open-source atlas containing molecular labels and definitions of anatomical regions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated extracellular signal–regulated kinase (ERK) as a readout of neural activity, we have developed a system to create and contextualize whole-brain maps of stimulus- and behavior-dependent neural activity. This mitogen-activated protein kinase (MAP)-mapping assay is technically simple, and data analysis is completely automated. Because MAP-mapping is performed on freely swimming fish, it is applicable to studies of nearly any stimulus or behavior. Here we demonstrate our high-throughput approach using pharmacological, visual and noxious stimuli, as well as hunting and feeding. The resultant maps outline hundreds of areas associated with behaviors. |
| 巻・号 | 12(11) |
| ページ | 1039-46 |
| 公開日 | 2015-11-1 |
| DOI | 10.1038/nmeth.3581 |
| PMID | 26778924 |
| PMC | PMC4710481 |
| MeSH | Algorithms Animals Automation Behavior, Animal Brain / metabolism* Brain / physiology Brain Mapping / methods Calcium / chemistry Extracellular Signal-Regulated MAP Kinases / metabolism* Image Processing, Computer-Assisted / methods* Immunohistochemistry Microscopy, Confocal Neurites / metabolism* Neurons / metabolism Neurons / physiology Phosphorylation Principal Component Analysis Reproducibility of Results Software Swimming Zebrafish |
| IF | 30.822 |
| 引用数 | 140 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg（isl1:GFP)/rw0 Tg(gad1b:GFP) |