RRC ID 39991
Author Barreiro-Iglesias A, Mysiak KS, Scott AL, Reimer MM, Yang Y, Becker CG, Becker T.
Title Serotonin Promotes Development and Regeneration of Spinal Motor Neurons in Zebrafish.
Journal Cell Rep
Abstract In contrast to mammals, zebrafish regenerate spinal motor neurons. During regeneration, developmental signals are re-deployed. Here, we show that, during development, diffuse serotonin promotes spinal motor neuron generation from pMN progenitor cells, leaving interneuron numbers unchanged. Pharmacological manipulations and receptor knockdown indicate that serotonin acts at least in part via 5-HT1A receptors. In adults, serotonin is supplied to the spinal cord mainly (90%) by descending axons from the brain. After a spinal lesion, serotonergic axons degenerate caudal to the lesion but sprout rostral to it. Toxin-mediated ablation of serotonergic axons also rostral to the lesion impaired regeneration of motor neurons only there. Conversely, intraperitoneal serotonin injections doubled numbers of new motor neurons and proliferating pMN-like progenitors caudal to the lesion. Regeneration of spinal-intrinsic serotonergic interneurons was unaltered by these manipulations. Hence, serotonin selectively promotes the development and adult regeneration of motor neurons in zebrafish.
Volume 13(5)
Pages 924-32
Published 2015-11-3
DOI 10.1016/j.celrep.2015.09.050
PII S2211-1247(15)01075-X
PMID 26565906
PMC PMC4635313
MeSH Animals Interneurons / cytology Interneurons / drug effects Interneurons / metabolism Motor Neurons / cytology Motor Neurons / drug effects Motor Neurons / metabolism* Nerve Regeneration* Neural Stem Cells / cytology Neural Stem Cells / drug effects Neural Stem Cells / metabolism Receptor, Serotonin, 5-HT1A / metabolism* Serotonin / metabolism* Serotonin / pharmacology Spinal Cord / cytology Spinal Cord / growth & development* Spinal Cord / physiology Zebrafish
IF 8.109
Times Cited 28
Zebrafish Tg(isl1:GFP)/rw0