RRC ID 40007
Author Taku AA, Marcaccio CL, Ye W, Krause GJ, Raper JA.
Title Attractant and repellent cues cooperate in guiding a subset of olfactory sensory axons to a well-defined protoglomerular target.
Journal Development
Abstract Olfactory sensory axons target well-defined intermediate targets in the zebrafish olfactory bulb called protoglomeruli well before they form odorant receptor-specific glomeruli. A subset of olfactory sensory neurons are labeled by expression of the or111-7:IRES:GAL4 transgene whose axons terminate in the central zone (CZ) protoglomerulus. Previous work has shown that some of these axons misproject to the more dorsal and anterior dorsal zone (DZ) protoglomerulus in the absence of Netrin 1/Dcc signaling. In search of additional cues that guide these axons to the CZ, we found that Semaphorin 3D (Sema3D) is expressed in the anterior bulb and acts as a repellent that pushes them towards the CZ. Further analysis indicates that Sema3D signaling is mediated through Nrp1a, while Nrp2b also promotes CZ targeting but in a Sema3D-independent manner. nrp1a, nrp2b and dcc transcripts are detected in or111-7 transgene-expressing neurons early in development and both Nrp1a and Dcc act cell-autonomously in sensory neurons to promote accurate targeting to the CZ. dcc and nrp1a double mutants have significantly more DZ misprojections than either single mutant, suggesting that the two signaling systems act independently and in parallel to direct a specific subset of sensory axons to their initial protoglomerular target.
Volume 143(1)
Pages 123-32
Published 2016-1-1
DOI 10.1242/dev.127985
PII 143/1/123
PMID 26732841
PMC PMC4725209
MeSH Animals Cues DCC Receptor DNA-Binding Proteins / metabolism Gene Expression Regulation, Developmental Nerve Growth Factors / genetics Nerve Growth Factors / metabolism Netrin-1 Neuropilin-1 / genetics Neuropilin-1 / metabolism* Neuropilin-2 / genetics Neuropilin-2 / metabolism* Olfactory Bulb / cytology* Olfactory Bulb / growth & development Olfactory Pathways / embryology* Olfactory Pathways / physiology Olfactory Receptor Neurons / cytology* Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Semaphorins / biosynthesis Semaphorins / genetics Semaphorins / metabolism Signal Transduction Transcription Factors / metabolism Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism Zebrafish / embryology* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 5.413
Times Cited 2
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Zebrafish rw037? or rw036?