| RRC ID |
4029
|
| 著者 |
Myouga F, Hosoda C, Umezawa T, Iizumi H, Kuromori T, Motohashi R, Shono Y, Nagata N, Ikeuchi M, Shinozaki K.
|
| タイトル |
A heterocomplex of iron superoxide dismutases defends chloroplast nucleoids against oxidative stress and is essential for chloroplast development in Arabidopsis.
|
| ジャーナル |
Plant Cell
|
| Abstract |
There are three iron superoxide dismutases in Arabidopsis thaliana: FE SUPEROXIDE DISMUTASE1 (FSD1), FSD2, and FSD3. Their biological roles in chloroplast development are unknown. Here, we show that FSD2 and FSD3 play essential roles in early chloroplast development, whereas FSD1, which is found in the cytoplasm, does not. An fsd2-1 fsd3-1 double mutant had a severe albino phenotype on agar plates, whereas fsd2 and fsd3 single knockout mutants had pale green phenotypes. Chloroplast development was arrested in young seedlings of the double mutant. The mutant plants were highly sensitive to oxidative stress and developed increased levels of reactive oxygen species (ROS) during extended darkness. The FSD2 and FSD3 proteins formed a heteromeric protein complex in the chloroplast nucleoids. Furthermore, transgenic Arabidopsis plants overexpressing both the FSD2 and FSD3 genes showed greater tolerance to oxidative stress induced by methyl viologen than did the wild type or single FSD2- or FSD3-overexpressing lines. We propose that heteromeric FSD2 and FSD3 act as ROS scavengers in the maintenance of early chloroplast development by protecting the chloroplast nucleoids from ROS.
|
| 巻・号 |
20(11)
|
| ページ |
3148-62
|
| 公開日 |
2008-11-1
|
| DOI |
10.1105/tpc.108.061341
|
| PII |
tpc.108.061341
|
| PMID |
18996978
|
| PMC |
PMC2613658
|
| MeSH |
Arabidopsis / genetics
Arabidopsis / growth & development*
Arabidopsis / metabolism
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Chloroplasts / genetics
Chloroplasts / metabolism*
Darkness
Gene Expression Regulation, Plant
Gene Knockout Techniques
Mutation
Oxidative Stress*
Phenotype
Plants, Genetically Modified / genetics
Plants, Genetically Modified / growth & development
Plants, Genetically Modified / metabolism
RNA, Plant / genetics
Reactive Oxygen Species / metabolism
Superoxide Dismutase / genetics
Superoxide Dismutase / metabolism*
|
| IF |
9.618
|
| 引用数 |
162
|
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda13279
pda03844
pda05750 |
