論文 - 詳細
| RRC ID | 40861 |
|---|---|
| 著者 | Li X, Jensen RL, Højberg O, Canibe N, Jensen BB. |
| タイトル | Olsenella scatoligenes sp. nov., a 3-methylindole- (skatole) and 4-methylphenol- (p-cresol) producing bacterium isolated from pig faeces. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
Strain SK9K4(T), which is a strictly anaerobic, non-motile, non-sporulating, Gram-stain-positive, saccharolytic coccobacillus, was isolated from pig faeces. SK9K4(T) metabolized indol-3-acetic acid to 3-methylindole (skatole), which is the main contributor to boar taint; it also produced 4-methylphenol (p-cresol) from p-hydroxyphenylacetic acid. Phylogenetic analyses, based on 16S rRNA gene sequences, revealed that the isolate represented a new lineage within the genus Olsenella of the family Atopobiaceae . Strain SK9K4(T) was most closely related to the type strains of the three species of the genus Olsenella with validly published names; Olsenella profusa DSM 13989(T) (93.6%), Olsenella uli DSM 7084(T) (93.5%) and Olsenella umbonata DSM 22620(T) (92.7%). DNA-DNA relatedness values of strain SK9K4(T) with O. profusa , O. uli and O. umbonata were 28.3%, 69.1% and 27.2%, respectively. The genomic DNA G+C content was 62.1 mol% and the major cellular fatty acids (constituting >10% of the total) were C(14 : 0) and C(18 : 1)ω9c. The major end product of glucose fermentation was lactic acid, with minor amounts of acetic acid and formic acid; no H2 was produced. Discrepancies in the fatty acid profiles, the MALDI-TOF mass spectra of cell extracts and the physiological and biochemical characteristics differentiated strain SK9K4(T) from other species of the genus Olsenella and indicate that the isolate represents a novel species within this genus. The name Olsenella scatoligenes sp. nov., is proposed and the type strain is SK9K4(T) ( = JCM 19907(T) = DSM 28304(T)). |
| 巻・号 | 65(Pt 4) |
| ページ | 1227-1233 |
| 公開日 | 2015-4-1 |
| DOI | 10.1099/ijs.0.000083 |
| PMID | 25634945 |
| MeSH | Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification Animals Bacterial Typing Techniques Base Composition Cresols / metabolism* DNA, Bacterial / genetics Fatty Acids / chemistry Feces / microbiology Fermentation Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Skatole / metabolism* Sus scrofa / microbiology* |
| IF | 2.415 |
| 引用数 | 17 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 一般微生物 | JCM 19907 |