| RRC ID |
40862
|
| Author |
Thamacharoensuk T, Kitahara M, Ohkuma M, Thongchul N, Tanasupawat S.
|
| Title |
Sporolactobacillus shoreae sp. nov. and Sporolactobacillus spathodeae sp. nov., two spore-forming lactic acid bacteria isolated from tree barks in Thailand.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Two Gram-stain-positive, endospore-forming lactic acid bacteria, designated BK92(T) and BK117-1(T), were isolated from tree barks in Thailand. Cells were catalase-negative and facultatively anaerobic rods. 16S rRNA gene sequence analysis indicated that these strains belonged to the genus Sporolactobacillus . Strains BK92(T) and BK117-1(T) showed the highest 16S rRNA gene sequence similarity to Sporolactobacillus putidus QC81-06(T) with 97.7% and 97.1% similarity, respectively. Analysis of phylogenetic relationships based on 16S rRNA and gyrB gene sequencing revealed that the positions of strains BK92(T) and BK117-1(T) were clearly separated from all related species of the genus Sporolactobacillus . Strains BK92(T) and BK117-1(T) had low DNA-DNA relatedness between each other and also with S. putidus QC81-06(T) and Sporolactobacillus vineae SL153(T). The DNA G+C content of strains BK92(T) and BK117-1(T) was 46.6 mol% and 47.4 mol%, respectively. The major fatty acids of strains BK92(T) and BK117-1(T) were anteiso-C(17 : 0) and anteiso-C(15 : 0). They contained meso-diaminopimelic acid in cell-wall peptidoglycan and had menaquinone with seven isoprene units (MK-7) as the predominant menaquinone. Based on evidence including phenotypic, genotypic and chemotaxonomic studies, strains BK92(T) and BK117-1(T) should be classified as representatives of novel species of the genus Sporolactobacillus , for which the names Sporolactobacillus shoreae sp. nov. and Sporolactobacillus spathodeae sp. nov. are proposed, respectively. The type strains are BK92(T) ( = JCM 19541(T) = LMG 28365(T) = PCU 336(T) = TISTR 2234(T)) and BK117-1(T) ( = JCM 19542(T) = LMG 28366(T) = PCU 337(T) = TISTR 2235(T)).
|
| Volume |
65(Pt 4)
|
| Pages |
1220-1226
|
| Published |
2015-4-1
|
| DOI |
10.1099/ijs.0.000084
|
| PMID |
25634946
|
| MeSH |
Bacillales / classification*
Bacillales / genetics
Bacillales / isolation & purification
Bacterial Typing Techniques
DNA, Bacterial / genetics
Diaminopimelic Acid / chemistry
Fatty Acids / chemistry
Genes, Bacterial
Lactic Acid
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny*
Plant Bark / microbiology*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Spores, Bacterial
Thailand
Trees / microbiology
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
|
| IF |
2.415
|
| Times Cited |
10
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 19541
JCM 19542
JCM 3516
JCM 15325
JCM 14637
JCM 6014
JCM 3514
JCM 3419
JCM 3417
JCM 2513 |
