| RRC ID |
41336
|
| Author |
Wu Q, Sun Y, Wang J, Lin X, Wang Y, Pegg LE, Fütterer A, Pfeffer K, Fu YX.
|
| Title |
Signal via lymphotoxin-beta R on bone marrow stromal cells is required for an early checkpoint of NK cell development.
|
| Journal |
J Immunol
|
| Abstract |
NK cells play an important role in the immune system but the cellular and molecular requirements for their early development are poorly understood. Lymphotoxin-alpha (LTalpha)(-/-) and LTbetaR(-/-) mice show a severe systemic reduction of NK cells, which provides an excellent model to study NK cell development. In this study, we show that the bone marrow (BM) or fetal liver cells from LTalpha(-/-) or LTbetaR(-/-) mice efficiently develop into mature NK cells in the presence of stromal cells from wild-type mice but not from LTalpha(-/-) or LTbetaR(-/-) mice. Direct activation of LTbetaR-expressing BM stromal cells is shown to promote to early NK cell development in vitro. Furthermore, the blockade of the interaction between LT and LTbetaR in adult wild-type mice by administration of LTbetaR-Ig impairs the development of NK cells in vivo. Together, these results indicate that the signal via LTbetaR on BM stromal cells by membrane LT is an important pathway for early NK cell development.
|
| Volume |
166(3)
|
| Pages |
1684-9
|
| Published |
2001-2-1
|
| DOI |
10.4049/jimmunol.166.3.1684
|
| PMID |
11160211
|
| MeSH |
Adoptive Transfer
Animals
Bone Marrow Cells / cytology
Bone Marrow Cells / immunology*
Bone Marrow Cells / metabolism
Bone Marrow Transplantation
Cell Differentiation / genetics
Cell Differentiation / immunology
Cell Line
Cells, Cultured
Hematopoietic Stem Cells / cytology*
Hematopoietic Stem Cells / immunology*
Hematopoietic Stem Cells / pathology
Homeostasis / immunology
Killer Cells, Natural / cytology*
Killer Cells, Natural / immunology*
Killer Cells, Natural / metabolism
Killer Cells, Natural / pathology
Lymphocyte Activation / genetics
Lymphocyte Count
Lymphoid Tissue / immunology
Lymphoid Tissue / pathology
Lymphopenia / genetics
Lymphopenia / immunology
Lymphopenia / therapy
Lymphotoxin beta Receptor
Lymphotoxin-alpha / genetics
Lymphotoxin-alpha / metabolism*
Lymphotoxin-beta
Membrane Proteins / metabolism*
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Tumor Necrosis Factor / deficiency
Receptors, Tumor Necrosis Factor / genetics
Receptors, Tumor Necrosis Factor / physiology*
Signal Transduction / genetics
Signal Transduction / immunology*
Stromal Cells / immunology
Stromal Cells / metabolism
T-Lymphocyte Subsets / pathology
|
| IF |
4.886
|
| Times Cited |
51
|
|
WOS Category
|
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
OP9(RCB1124) |
