| RRC ID |
41391
|
| 著者 |
Kuwahara M, Takeda M, Takeuchi Y, Kuwahara M, Harada T, Maita K.
|
| タイトル |
Transforming growth factor beta production by spontaneous malignant mesothelioma cell lines derived from Fisher 344 rats.
|
| ジャーナル |
Virchows Arch
|
| Abstract |
We investigated whether transforming growth factor beta (TGF-beta) is involved in the growth of malignant mesothelioma (MM) cells in culture. TGF-beta production was examined in two mesothelioma cell lines (MeET-4 and -6) that were established from rat spontaneous MM in our laboratory. TGF-beta bioactivity in conditioned medium of these cell lines was analyzed using a CCL64 mink lung epithelial cell growth inhibition assay and found to be 30-70 times higher than that of normal rat mesothelial cells (MCs). The MM cell lines also showed considerably higher levels of TGF-beta mRNA expression when compared with MCs. The bioactivity and mRNA expression level were greater in MeET-4 than MeET-6. When MeET-4 was treated with antisense TGF-beta1 oligonucleotide (ODN), a significant decrease in both anchorage-dependent and -independent growth was observed. Treatment with exogenous TGF-beta resulted in no effects on the growth pattern of the MM cell lines, while proliferation of the MCs was slightly induced. It is considered that TGF-beta appears to be produced by rat spontaneous MM cells through an autocrine mechanism and could modulate the malignant growth of the tumor cells.
|
| 巻・号 |
438(5)
|
| ページ |
492-7
|
| 公開日 |
2001-5-1
|
| DOI |
10.1007/s004280000363
|
| PMID |
11407478
|
| MeSH |
Animals
Cell Count
Cell Division / drug effects
Culture Media, Conditioned / pharmacology
DNA Primers / chemistry
Dose-Response Relationship, Drug
Epithelium / drug effects
Mesothelioma / genetics
Mesothelioma / metabolism*
Mesothelioma / pathology
Molecular Sequence Data
Oligonucleotides, Antisense / pharmacology
Peritoneum / cytology
Polymerase Chain Reaction
RNA, Messenger / biosynthesis
RNA, Neoplasm / biosynthesis
Rats
Rats, Inbred F344
Retroperitoneal Neoplasms / genetics
Retroperitoneal Neoplasms / metabolism*
Retroperitoneal Neoplasms / pathology
Transforming Growth Factor beta / biosynthesis*
Transforming Growth Factor beta / genetics
Tumor Cells, Cultured / metabolism*
Tumor Cells, Cultured / pathology
|
| IF |
2.906
|
| 引用数 |
11
|
|
WOS 分野
|
PATHOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Mv.1.Lu(NBL-7)(RCB0996) |
