Reference - Detail
| RRC ID | 41411 |
|---|---|
| Author | Maruyama Y, Suzuki Y, Kazusaka A, Fujita S. |
| Title | Uptake of the dopaminergic neurotoxin, norsalsolinol, into PC12 cells via dopamine transporter. |
| Journal | Arch Toxicol |
| Abstract |
The uptake of norsalsolinol, a neurotoxin candidate causing parkinsonism-like symptoms, was studied in PC12 cells. The compound was actively taken up by the PC12 cells, with a Km value of 176.24 +/-9.1 microM and a maximum velocity of 55.6 +/- 7.0 pmol/min per mg protein; norsalsolinol uptake was dependent on the presence of extracellular Na+. The uptake of norsalsolinol was sensitive to two dopamine transporter inhibitors, GBR-12909 and reserpine, but was less sensitive to desipramine, a noradrenaline transporter inhibitor. Dopamine competitively inhibited norsalsolinol uptake into PC12 cells with a Ki value of 271.2 +/- 61.6 microM. These results suggest that norsalsolinol is taken up into PC12 cells mainly by the dopamine transporter. |
| Volume | 75(4) |
| Pages | 209-13 |
| Published | 2001-6-1 |
| DOI | 10.1007/s002040000202 |
| PMID | 11482518 |
| MeSH | Adrenergic Uptake Inhibitors / pharmacology Animals Cells, Cultured Chromatography, High Pressure Liquid Desipramine / pharmacology Dopamine / metabolism* Dopamine Plasma Membrane Transport Proteins Dopamine Uptake Inhibitors / pharmacology Dose-Response Relationship, Drug Membrane Glycoproteins* Membrane Transport Proteins / metabolism* Nerve Tissue Proteins* PC12 Cells Piperazines / pharmacology Rats Reserpine / pharmacology Reverse Transcriptase Polymerase Chain Reaction Salsoline Alkaloids / metabolism* Salsoline Alkaloids / toxicity |
| IF | 5.059 |
| Times Cited | 2 |
| WOS Category | TOXICOLOGY |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |