論文 - 詳細
| RRC ID | 41448 |
|---|---|
| 著者 | Kamiya H, Murata-Kamiya N, Karino N, Ueno Y, Matsuda A, Kasai H. |
| タイトル | Induction of T --> G and T --> A transversions by 5-formyluracil in mammalian cells. |
| ジャーナル | Mutat Res |
| Abstract |
Oxidatively damaged thymine, 5-formyluracil (5-fU), was incorporated into a predetermined site of double-stranded shuttle vectors. The nucleotide sequences in which the modified base was incorporated were 5'-CFTAAG-3' and 5'-CTFAAG-3' (F represents 5-fU), the recognition site for the restriction enzyme AflII (5'-CTTAAG-3'). The 5-fU was incorporated into a template strand of either the leading or lagging strand of DNA replication. The modified DNAs were transfected into simian COS-7 cells, and the DNAs replicated in the cells were recovered and were analyzed after the second transfection into Escherichia coli. The 5-fU did not block DNA replication in mammalian cells. The 5-fU residues were weakly mutagenic, and their mutation frequencies in double-stranded vectors were 0.01-0.04%. The T --> G and T --> A transversions were the mutations found most frequently, suggesting the formation of 5-fU.C and 5-fU.T base pairs, respectively. This is the first report that clearly shows the induction of transversion mutations by an oxidized pyrimidine base in DNA in mammalian cells. |
| 巻・号 | 513(1-2) |
| ページ | 213-22 |
| 公開日 | 2002-1-15 |
| DOI | 10.1016/s1383-5718(01)00312-6 |
| PII | S1383571801003126 |
| PMID | 11719107 |
| MeSH | Animals COS Cells Molecular Conformation Mutagens / toxicity* Mutation Uracil / analogs & derivatives* Uracil / chemistry Uracil / toxicity* |
| IF | 2.463 |
| 引用数 | 37 |
| WOS 分野 | TOXICOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |